Detailed information of TA system TA227075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2498662..2499282
• Replicon: chromosome
• Accession: NZ_CP088282
• Organism: Bradyrhizobium quebecense strain 12S5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J4P68_RS12250
• Protein ID: WP_207832818.1
• Coordinates: 2498662..2498958 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J4P68_RS12255
• Protein ID: WP_176529221.1
• Coordinates: 2498974..2499282 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4P68_RS12230 (J4P68_0012230)	2494466..2494951	+	486	WP_224924592.1	antibiotic biosynthesis monooxygenase	-
J4P68_RS12235 (J4P68_0012235)	2494996..2496141	-	1146	WP_224924594.1	acyltransferase	-
J4P68_RS12240 (J4P68_0012240)	2496434..2497927	-	1494	WP_176529223.1	FAD-linked oxidase C-terminal domain-containing protein	-
J4P68_RS12245 (J4P68_0012245)	2498176..2498568	+	393	WP_092124037.1	cytochrome c family protein	-
J4P68_RS12250 (J4P68_0012250)	2498662..2498958	+	297	WP_207832818.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J4P68_RS12255 (J4P68_0012255)	2498974..2499282	+	309	WP_176529221.1	HigA family addiction module antitoxin	Antitoxin
J4P68_RS12260 (J4P68_0012260)	2499302..2501038	-	1737	WP_207832820.1	ubiquinone-dependent pyruvate dehydrogenase	-
J4P68_RS12265 (J4P68_0012265)	2501122..2502105	-	984	WP_207832822.1	alpha/beta hydrolase	-
J4P68_RS12270 (J4P68_0012270)	2502471..2502647	+	177	WP_167351865.1	hypothetical protein	-
J4P68_RS12275 (J4P68_0012275)	2502724..2503314	-	591	WP_173640588.1	tetratricopeptide repeat protein	-
J4P68_RS12280 (J4P68_0012280)	2503501..2503626	-	126	WP_002718645.1	type B 50S ribosomal protein L36	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-96)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-84)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11594.08 Da
• Isoelectric Point: 9.7733

>T227075 WP_207832818.1 NZ_CP088282:2498662-2498958 [Bradyrhizobium quebecense]
MILSFRDEWLRAFFTEDAKSRNIPSDLEDRLFRKLQMLDDATTDQDLRVPPSNHFEKLRGNLAGLHSIRVNKQWRLVFRW
SGARGETQGVYLGDHSYR

Nucleotide

• Length: 297 bp

>T227075 NZ_CP122712:c1205421-1205044 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCGGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTTAC
CAGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACGCCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AACGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTGAACAATATCACCCGGGGTAAACATTCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11486.33 Da
• Isoelectric Point: 11.4325

>AT227075 WP_176529221.1 NZ_CP088282:2498974-2499282 [Bradyrhizobium quebecense]
MLTTKRKPATLGEILREEFMAPLGLTQGALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPEFWLNVQRRTDLW
QAMNSPSERKRIERAKPLGRAA

Nucleotide

• Length: 309 bp

>AT227075 NZ_CP122712:c1205879-1205511 [Escherichia coli]
GTGTCAGACTCGCGCCATGAGACAAATTATCCCGACGACCATAACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAAGGCAATCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGTCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA