Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3329495..3330222 Replicon chromosome
Accession NC_010473
Organism Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ECDH10B_RS16565 Protein ID WP_000550189.1
Coordinates 3329908..3330222 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ECDH10B_RS16560 Protein ID WP_000560266.1
Coordinates 3329495..3329911 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH10B_RS16550 (ECDH10B_3255) 3324655..3327006 + 2352 WP_000695487.1 alpha-glucosidase -
ECDH10B_RS16555 (ECDH10B_3256) 3327432..3329450 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ECDH10B_RS16560 (ECDH10B_3257) 3329495..3329911 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ECDH10B_RS16565 (ECDH10B_3258) 3329908..3330222 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ECDH10B_RS16575 (ECDH10B_3259) 3330506..3331642 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ECDH10B_RS16580 (ECDH10B_3260) 3331727..3332230 + 504 WP_001333820.1 M48 family metallopeptidase -
ECDH10B_RS16585 (ECDH10B_3261) 3332307..3332999 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
ECDH10B_RS16590 (ECDH10B_3262) 3333078..3334064 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T22702 WP_000550189.1 NC_010473:c3330222-3329908 [Escherichia coli str. K-12 substr. DH10B]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T22702 NC_010473:c3330222-3329908 [Escherichia coli str. K-12 substr. DH10B]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT22702 WP_000560266.1 NC_010473:c3329911-3329495 [Escherichia coli str. K-12 substr. DH10B]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT22702 NC_010473:c3329911-3329495 [Escherichia coli str. K-12 substr. DH10B]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References