Detailed information of TA system TA227010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 5198888..5199408
• Replicon: chromosome
• Accession: NZ_CP088247
• Organism: Methylorubrum sp. B1-46

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LPC10_RS24110
• Protein ID: WP_231344767.1
• Coordinates: 5199139..5199408 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LPC10_RS24105
• Protein ID: WP_231347138.1
• Coordinates: 5198888..5199142 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPC10_RS24090 (LPC10_24090)	5194294..5196306	-	2013	WP_231344763.1	methyl-accepting chemotaxis protein	-
LPC10_RS24095 (LPC10_24095)	5196751..5198139	-	1389	WP_231344764.1	cysteine--tRNA ligase	-
LPC10_RS24100 (LPC10_24100)	5198468..5198794	+	327	WP_231344766.1	hypothetical protein	-
LPC10_RS24105 (LPC10_24105)	5198888..5199142	+	255	WP_231347138.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LPC10_RS24110 (LPC10_24110)	5199139..5199408	+	270	WP_231344767.1	Txe/YoeB family addiction module toxin	Toxin
LPC10_RS24115 (LPC10_24115)	5199431..5199976	-	546	WP_231344769.1	CreA family protein	-
LPC10_RS24120 (LPC10_24120)	5200050..5201135	+	1086	WP_231344770.1	DUF2865 domain-containing protein	-
LPC10_RS24125 (LPC10_24125)	5201296..5203392	+	2097	WP_231344771.1	OmpA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-72)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10491.90 Da
• Isoelectric Point: 9.5455

>T227010 WP_231344767.1 NZ_CP088247:5199139-5199408 [Methylorubrum sp. B1-46]
VRLLWSDQAWEDYLYWQTTEPGTLRRLNELIKDASRSPFTGLGKPEPLKGNLKGWWSRRITGSHRLVYRVGGMGTEQTLE
IAQCRWHYE

Nucleotide

• Length: 270 bp

>T227010 NZ_CP122679:1998312-1998563 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9288.47 Da
• Isoelectric Point: 4.8615

>AT227010 WP_231347138.1 NZ_CP088247:5198888-5199142 [Methylorubrum sp. B1-46]
MGYVGYTELRRNLAEHLDRVESDRAELVVSRRNREDLAIIPLAELESLRETLHLLGTPANAARLIAGVRQLDAGGGAEHS
LIEE

Nucleotide

• Length: 255 bp

>AT227010 NZ_CP122679:1998560-1998814 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA