Detailed information of TA system TA022701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3263618..3264311
• Replicon: chromosome
• Accession: NC_010473
• Organism: Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: ECDH10B_RS16240
• Protein ID: WP_000415584.1
• Coordinates: 3264015..3264311 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: ECDH10B_RS16235
• Protein ID: WP_000650107.1
• Coordinates: 3263618..3264013 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECDH10B_RS16225 (ECDH10B_3193)	3259482..3261740	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
ECDH10B_RS16230 (ECDH10B_3194)	3261878..3263485	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
ECDH10B_RS16235 (ECDH10B_3195)	3263618..3264013	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
ECDH10B_RS16240 (ECDH10B_3196)	3264015..3264311	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
ECDH10B_RS16245 (ECDH10B_3197)	3264516..3264998	-	483	WP_000183505.1	transcriptional regulator	-
ECDH10B_RS16250 (ECDH10B_3198)	3265051..3265443	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
ECDH10B_RS16255 (ECDH10B_3199)	3265595..3266254	+	660	WP_001221495.1	two-component system response regulator QseB	-
ECDH10B_RS16260 (ECDH10B_3200)	3266251..3267600	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
ECDH10B_RS16265 (ECDH10B_3201)	3267646..3267978	-	333	WP_000917684.1	DUF2645 family protein	-
ECDH10B_RS16270 (ECDH10B_3202)	3268297..3268878	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
ECDH10B_RS16275 (ECDH10B_3203)	3268909..3269223	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T22701 WP_000415584.1 NC_010473:c3264311-3264015 [Escherichia coli str. K-12 substr. DH10B]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T22701 NC_010473:c3264311-3264015 [Escherichia coli str. K-12 substr. DH10B]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT22701 WP_000650107.1 NC_010473:c3264013-3263618 [Escherichia coli str. K-12 substr. DH10B]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT22701 NC_010473:c3264013-3263618 [Escherichia coli str. K-12 substr. DH10B]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65