Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3983205..3983793 Replicon chromosome
Accession NZ_CP088244
Organism Stenotrophomonas maltophilia strain 2013-SM4

Toxin (Protein)


Gene name higB Uniprot ID A0A149QBL6
Locus tag LQ335_RS18245 Protein ID WP_061479868.1
Coordinates 3983515..3983793 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A149QBV7
Locus tag LQ335_RS18240 Protein ID WP_061479867.1
Coordinates 3983205..3983504 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ335_RS18220 (LQ335_18220) 3978646..3979830 + 1185 WP_061479706.1 pyridoxal phosphate-dependent aminotransferase -
LQ335_RS18225 (LQ335_18225) 3979940..3982036 - 2097 WP_134968368.1 prolyl oligopeptidase family serine peptidase -
LQ335_RS18230 (LQ335_18230) 3982106..3982405 - 300 WP_061479865.1 hypothetical protein -
LQ335_RS18235 (LQ335_18235) 3982534..3983133 + 600 WP_134968216.1 lipocalin family protein -
LQ335_RS18240 (LQ335_18240) 3983205..3983504 - 300 WP_061479867.1 HigA family addiction module antitoxin Antitoxin
LQ335_RS18245 (LQ335_18245) 3983515..3983793 - 279 WP_061479868.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LQ335_RS18250 (LQ335_18250) 3983954..3984643 - 690 WP_065196496.1 methionine ABC transporter permease -
LQ335_RS18255 (LQ335_18255) 3984640..3985647 - 1008 WP_005419464.1 methionine ABC transporter ATP-binding protein -
LQ335_RS18260 (LQ335_18260) 3985827..3986723 - 897 WP_019660712.1 DMT family transporter -
LQ335_RS18265 (LQ335_18265) 3986877..3987359 + 483 WP_005419468.1 YajQ family cyclic di-GMP-binding protein -
LQ335_RS18270 (LQ335_18270) 3987460..3988026 + 567 WP_061479870.1 DUF1415 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-68)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10731.37 Da        Isoelectric Point: 10.5817

>T227004 WP_061479868.1 NZ_CP088244:c3983793-3983515 [Stenotrophomonas maltophilia]
MIRSFRCKHTRALFEGHCVRAWLPIRRAAERKLQMLDSAQTLAFLRSPPGNRLEALAGDRKGQHSLRINDQWRLCFIWTD
SGPQSVEIVDYH

Download         Length: 279 bp

>T227004 NZ_CP122676:c4861384-4861073 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10888.52 Da        Isoelectric Point: 10.1924

>AT227004 WP_061479867.1 NZ_CP088244:c3983504-3983205 [Stenotrophomonas maltophilia]
MKPTNNRMRAIHPGEILREEFLSPLALSVNALARELDVPATRLHAIVHGERAITADTAARLARHFGGDAASWLALQATYD
LKTLPTLGEIERRVQPRAA

Download         Length: 300 bp

>AT227004 NZ_CP122676:c4861072-4860782 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A149QBL6

Antitoxin

Source ID Structure
AlphaFold DB A0A149QBV7

References