Detailed information of TA system TA227002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1257512..1258100
• Replicon: chromosome
• Accession: NZ_CP088243
• Organism: Stenotrophomonas maltophilia strain 2013-SM12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LQ332_RS05610
• Protein ID: WP_080355515.1
• Coordinates: 1257822..1258100 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8E3RGU6
• Locus tag: LQ332_RS05605
• Protein ID: WP_049439995.1
• Coordinates: 1257512..1257811 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ332_RS05585 (LQ332_05585)	1252939..1254126	+	1188	WP_130768214.1	pyridoxal phosphate-dependent aminotransferase	-
LQ332_RS05590 (LQ332_05590)	1254251..1256347	-	2097	WP_064239222.1	prolyl oligopeptidase family serine peptidase	-
LQ332_RS05595 (LQ332_05595)	1256417..1256740	-	324	WP_103282036.1	hypothetical protein	-
LQ332_RS05600 (LQ332_05600)	1256869..1257471	+	603	WP_049464637.1	lipocalin family protein	-
LQ332_RS05605 (LQ332_05605)	1257512..1257811	-	300	WP_049439995.1	HigA family addiction module antitoxin	Antitoxin
LQ332_RS05610 (LQ332_05610)	1257822..1258100	-	279	WP_080355515.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LQ332_RS05615 (LQ332_05615)	1258307..1258996	-	690	WP_103282037.1	methionine ABC transporter permease	-
LQ332_RS05620 (LQ332_05620)	1258993..1260000	-	1008	WP_010481369.1	methionine ABC transporter ATP-binding protein	-
LQ332_RS05625 (LQ332_05625)	1260211..1261107	-	897	WP_130768215.1	DMT family transporter	-
LQ332_RS05630 (LQ332_05630)	1261203..1261685	+	483	WP_049464641.1	YajQ family cyclic di-GMP-binding protein	-
LQ332_RS05635 (LQ332_05635)	1261720..1262817	-	1098	WP_049464643.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (27-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10682.19 Da
• Isoelectric Point: 10.4115

>T227002 WP_080355515.1 NZ_CP088243:c1258100-1257822 [Stenotrophomonas maltophilia]
MIRSFRCKQTRALFEGACPRAWRPIRRAAERKLQLLDSAQTLAFLRSPPGNRLEALSGDRRGQYSLRINDQWRLCFTWTD
SGADAVEIVDYH

Nucleotide

• Length: 279 bp

>T227002 NZ_CP122676:4550003-4550380 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCGGTTTACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTTAC
CAGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACGCCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATATCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACAAGCCGGGATTC
AACGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTGAACAATATCACCCGGGGTAAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10885.59 Da
• Isoelectric Point: 11.3450

>AT227002 WP_049439995.1 NZ_CP088243:c1257811-1257512 [Stenotrophomonas maltophilia]
MKRPHNGMRAIHPGEILREEFLLPLSLSVNALAKALDVPATRLHAIVHGQRSISADTAARLARHFGGDAASWLAMQAAYD
LKTLPTRSEIERRVQPRAA

Nucleotide

• Length: 300 bp

>AT227002 NZ_CP122676:4549545-4549913 [Escherichia coli]
GTGTCAGACACACTCCATGAAACAAATTATCCCGACGACAACAACGACCGCTCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCAGGGCACGTCTGGTGCAGGAAGGGAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGTCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA