Detailed information of TA system TA226986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1149951..1150600
• Replicon: chromosome
• Accession: NZ_CP088229
• Organism: Enterobacter kobei strain Ek72

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LK774_RS05425
• Protein ID: WP_023332294.1
• Coordinates: 1150238..1150600 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2W0GCS7
• Locus tag: LK774_RS05420
• Protein ID: WP_014882863.1
• Coordinates: 1149951..1150250 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK774_RS05410 (LK774_05425)	1146376..1148349	+	1974	WP_023332292.1	PhoX family phosphatase	-
LK774_RS05415 (LK774_05430)	1148452..1149840	+	1389	WP_023332293.1	phenylalanine transporter	-
LK774_RS05420 (LK774_05435)	1149951..1150250	-	300	WP_014882863.1	helix-turn-helix transcriptional regulator	Antitoxin
LK774_RS05425 (LK774_05440)	1150238..1150600	-	363	WP_023332294.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LK774_RS05430 (LK774_05445)	1150806..1152041	+	1236	WP_023332295.1	MFS transporter	-
LK774_RS05435 (LK774_05450)	1152057..1153082	+	1026	WP_023332296.1	sugar phosphate isomerase/epimerase	-
LK774_RS05440 (LK774_05455)	1153075..1154217	+	1143	WP_249979714.1	Gfo/Idh/MocA family oxidoreductase	-
LK774_RS05445 (LK774_05460)	1154293..1155264	+	972	WP_014882868.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-80)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13953.90 Da
• Isoelectric Point: 6.2260

>T226986 WP_023332294.1 NZ_CP088229:c1150600-1150238 [Enterobacter kobei]
MWNVETTDRFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Nucleotide

• Length: 363 bp

>T226986 NZ_CP122676:911665-912072 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10860.61 Da
• Isoelectric Point: 9.8627

>AT226986 WP_014882863.1 NZ_CP088229:c1150250-1149951 [Enterobacter kobei]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT226986 NZ_CP122676:911418-911684 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2W0GCS7