Detailed information of TA system TA226983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31715..32445
• Replicon: chromosome
• Accession: NZ_CP088229
• Organism: Enterobacter kobei strain Ek72

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W0GI63
• Locus tag: LK774_RS00150
• Protein ID: WP_023331686.1
• Coordinates: 31715..32029 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LK774_RS00155
• Protein ID: WP_023331687.1
• Coordinates: 32026..32445 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK774_RS00130 (LK774_00130)	27380..28468	+	1089	WP_023331681.1	cellulase family glycosylhydrolase	-
LK774_RS00135 (LK774_00135)	28371..29555	-	1185	WP_032629627.1	multidrug efflux MFS transporter EmrD	-
LK774_RS00140 (LK774_00140)	29731..30564	-	834	WP_023331683.1	EamA family transporter	-
LK774_RS00145 (LK774_00145)	30627..31073	-	447	WP_023331684.1	GNAT family N-acetyltransferase	-
LK774_RS00150 (LK774_00155)	31715..32029	+	315	WP_023331686.1	type II toxin-antitoxin system HigB family toxin	Toxin
LK774_RS00155 (LK774_00160)	32026..32445	+	420	WP_023331687.1	helix-turn-helix domain-containing protein	Antitoxin
LK774_RS00160 (LK774_00165)	32595..32693	+	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
LK774_RS00165 (LK774_00170)	32800..34488	+	1689	WP_023331689.1	acetolactate synthase large subunit	-
LK774_RS00170 (LK774_00175)	34492..34779	+	288	WP_010426528.1	acetolactate synthase small subunit	-
LK774_RS00175 (LK774_00180)	34862..35455	+	594	WP_014881931.1	transcriptional regulator UhpA	-
LK774_RS00180 (LK774_00185)	35452..36957	+	1506	WP_023331690.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.59 Da
• Isoelectric Point: 10.2825

>T226983 WP_023331686.1 NZ_CP088229:31715-32029 [Enterobacter kobei]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T226983 NZ_CP122673:c4861388-4861077 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15561.18 Da
• Isoelectric Point: 5.6761

>AT226983 WP_023331687.1 NZ_CP088229:32026-32445 [Enterobacter kobei]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT226983 NZ_CP122673:c4861076-4860786 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A2W0GI63