Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1216214..1216941 Replicon chromosome
Accession NZ_CP088221
Organism Enterobacter hormaechei strain Eh60

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag LK764_RS05800 Protein ID WP_022650547.1
Coordinates 1216630..1216941 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3V464
Locus tag LK764_RS05795 Protein ID WP_022650546.1
Coordinates 1216214..1216633 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LK764_RS05780 (LK764_05800) 1213286..1214407 - 1122 WP_023324277.1 YbdK family carboxylate-amine ligase -
LK764_RS05785 (LK764_05805) 1214513..1215403 + 891 WP_126525253.1 oxidoreductase -
LK764_RS05790 (LK764_05810) 1215456..1215890 - 435 WP_045342299.1 hypothetical protein -
LK764_RS05795 (LK764_05815) 1216214..1216633 - 420 WP_022650546.1 helix-turn-helix domain-containing protein Antitoxin
LK764_RS05800 (LK764_05820) 1216630..1216941 - 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
LK764_RS05805 (LK764_05825) 1217397..1218053 + 657 WP_022650548.1 hypothetical protein -
LK764_RS05810 (LK764_05830) 1218166..1218621 - 456 WP_220153636.1 hypothetical protein -
LK764_RS05815 (LK764_05835) 1218904..1219785 - 882 WP_022650550.1 LysR family transcriptional regulator -
LK764_RS05820 (LK764_05840) 1219891..1220670 + 780 WP_026094386.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T226967 WP_022650547.1 NZ_CP088221:c1216941-1216630 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T226967 NZ_CP122673:1969815-1970066 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15447.51 Da        Isoelectric Point: 4.6051

>AT226967 WP_022650546.1 NZ_CP088221:c1216633-1216214 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT226967 NZ_CP122673:1970063-1970317 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3V464

References