Detailed information of TA system TA226810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4698910..4699512
• Replicon: chromosome
• Accession: NZ_CP088138
• Organism: Salmonella enterica subsp. enterica serovar Hissar strain SCPM-O-B-4549

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: LRM92_RS22925
• Protein ID: WP_001159630.1
• Coordinates: 4699201..4699512 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRM92_RS22920
• Protein ID: WP_000362050.1
• Coordinates: 4698910..4699200 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM92_RS22905 (4696403)	4696403..4697305	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LRM92_RS22910 (4697302)	4697302..4697937	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LRM92_RS22915 (4697934)	4697934..4698863	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LRM92_RS22920 (4698910)	4698910..4699200	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
LRM92_RS22925 (4699201)	4699201..4699512	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
LRM92_RS22930 (4699730)	4699730..4700659	+	930	WP_001127703.1	alpha/beta hydrolase	-
LRM92_RS22935 (4700745)	4700745..4701056	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LRM92_RS22940 (4701053)	4701053..4701499	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LRM92_RS22945 (4701514)	4701514..4702455	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LRM92_RS22950 (4702500)	4702500..4702937	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
LRM92_RS22955 (4702934)	4702934..4703806	-	873	WP_000921427.1	virulence factor BrkB family protein	-
LRM92_RS22960 (4703800)	4703800..4704399	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T226810 WP_001159630.1 NZ_CP088138:c4699512-4699201 [Salmonella enterica subsp. enterica serovar Hissar]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T226810 NZ_CP122629:c4502096-4501923 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT226810 WP_000362050.1 NZ_CP088138:c4699200-4698910 [Salmonella enterica subsp. enterica serovar Hissar]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT226810 NZ_CP122629:c4502420-4502124 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0R9NS64