Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 226109..226803 Replicon chromosome
Accession NC_010473
Organism Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag ECDH10B_RS01085 Protein ID WP_001263489.1
Coordinates 226405..226803 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag ECDH10B_RS01080 Protein ID WP_000554758.1
Coordinates 226109..226402 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH10B_RS01060 (ECDH10B_0210) 221741..222238 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ECDH10B_RS01065 (ECDH10B_0211) 222462..224174 - 1713 Protein_202 flagellar biosynthesis protein FlhA -
ECDH10B_RS01070 (ECDH10B_0212) 224146..224931 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
ECDH10B_RS01075 (ECDH10B_0213) 225002..226057 + 1056 WP_001226164.1 DNA polymerase IV -
ECDH10B_RS01080 (ECDH10B_0214) 226109..226402 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ECDH10B_RS01085 (ECDH10B_0215) 226405..226803 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ECDH10B_RS01090 (ECDH10B_0216) 226813..227265 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
ECDH10B_RS01095 (ECDH10B_0217) 227583..227789 + 207 Protein_208 RtcB family protein -
ECDH10B_RS01100 (ECDH10B_0218) 227785..228306 + 522 Protein_209 peptide chain release factor H -
ECDH10B_RS01105 (ECDH10B_0219) 228363..229820 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
ECDH10B_RS01110 (ECDH10B_0220) 230081..230539 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T22677 WP_001263489.1 NC_010473:226405-226803 [Escherichia coli str. K-12 substr. DH10B]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T22677 NC_010473:226405-226803 [Escherichia coli str. K-12 substr. DH10B]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT22677 WP_000554758.1 NC_010473:226109-226402 [Escherichia coli str. K-12 substr. DH10B]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT22677 NC_010473:226109-226402 [Escherichia coli str. K-12 substr. DH10B]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References