Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 220065..220606 Replicon chromosome
Accession NC_010473
Organism Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag ECDH10B_RS01045 Protein ID WP_000615983.1
Coordinates 220065..220343 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag ECDH10B_RS01050 Protein ID WP_000729703.1
Coordinates 220346..220606 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH10B_RS01030 (ECDH10B_0204) 217647..218225 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
ECDH10B_RS01035 (ECDH10B_0205) 218431..219198 + 768 WP_012305459.1 class II glutamine amidotransferase -
ECDH10B_RS01040 (ECDH10B_0206) 219169..219909 - 741 WP_001225679.1 murein L,D-transpeptidase -
ECDH10B_RS01045 (ECDH10B_0207) 220065..220343 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ECDH10B_RS01050 (ECDH10B_0208) 220346..220606 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECDH10B_RS01055 (ECDH10B_0209) 220816..221565 + 750 WP_000056849.1 C40 family peptidase -
ECDH10B_RS01060 (ECDH10B_0210) 221741..222238 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ECDH10B_RS01065 (ECDH10B_0211) 222462..224174 - 1713 Protein_202 flagellar biosynthesis protein FlhA -
ECDH10B_RS01070 (ECDH10B_0212) 224146..224931 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T22676 WP_000615983.1 NC_010473:c220343-220065 [Escherichia coli str. K-12 substr. DH10B]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T22676 NC_010473:c220343-220065 [Escherichia coli str. K-12 substr. DH10B]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT22676 WP_000729703.1 NC_010473:c220606-220346 [Escherichia coli str. K-12 substr. DH10B]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT22676 NC_010473:c220606-220346 [Escherichia coli str. K-12 substr. DH10B]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References