Detailed information of TA system TA022676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 220065..220606
• Replicon: chromosome
• Accession: NC_010473
• Organism: Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q47149
• Locus tag: ECDH10B_RS01045
• Protein ID: WP_000615983.1
• Coordinates: 220065..220343 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: Q47150
• Locus tag: ECDH10B_RS01050
• Protein ID: WP_000729703.1
• Coordinates: 220346..220606 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECDH10B_RS01030 (ECDH10B_0204)	217647..218225	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
ECDH10B_RS01035 (ECDH10B_0205)	218431..219198	+	768	WP_012305459.1	class II glutamine amidotransferase	-
ECDH10B_RS01040 (ECDH10B_0206)	219169..219909	-	741	WP_001225679.1	murein L,D-transpeptidase	-
ECDH10B_RS01045 (ECDH10B_0207)	220065..220343	-	279	WP_000615983.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
ECDH10B_RS01050 (ECDH10B_0208)	220346..220606	-	261	WP_000729703.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
ECDH10B_RS01055 (ECDH10B_0209)	220816..221565	+	750	WP_000056849.1	C40 family peptidase	-
ECDH10B_RS01060 (ECDH10B_0210)	221741..222238	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
ECDH10B_RS01065 (ECDH10B_0211)	222462..224174	-	1713	Protein_202	flagellar biosynthesis protein FlhA	-
ECDH10B_RS01070 (ECDH10B_0212)	224146..224931	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10846.62 Da
• Isoelectric Point: 9.9941

>T22676 WP_000615983.1 NC_010473:c220343-220065 [Escherichia coli str. K-12 substr. DH10B]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T22676 NC_010473:c220343-220065 [Escherichia coli str. K-12 substr. DH10B]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9405.80 Da
• Isoelectric Point: 5.0758

>AT22676 WP_000729703.1 NC_010473:c220606-220346 [Escherichia coli str. K-12 substr. DH10B]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT22676 NC_010473:c220606-220346 [Escherichia coli str. K-12 substr. DH10B]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 4Q2U

Antitoxin

• AlphaFold DB: A0A5F1F716