Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3742072..3742613 Replicon chromosome
Accession NZ_CP088131
Organism Escherichia coli strain 81

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag LRM32_RS18380 Protein ID WP_000615979.1
Coordinates 3742335..3742613 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LRM32_RS18375 Protein ID WP_000729704.1
Coordinates 3742072..3742332 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM32_RS18355 (3737755) 3737755..3738540 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
LRM32_RS18360 (3738512) 3738512..3740224 + 1713 Protein_3607 flagellar biosynthesis protein FlhA -
LRM32_RS18365 (3740440) 3740440..3740937 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
LRM32_RS18370 (3741113) 3741113..3741886 - 774 WP_001353765.1 C40 family peptidase -
LRM32_RS18375 (3742072) 3742072..3742332 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LRM32_RS18380 (3742335) 3742335..3742613 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LRM32_RS18385 (3742769) 3742769..3743509 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LRM32_RS18390 (3743480) 3743480..3744247 - 768 WP_000333379.1 class II glutamine amidotransferase -
LRM32_RS18395 (3744453) 3744453..3745031 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T226736 WP_000615979.1 NZ_CP088131:3742335-3742613 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T226736 NZ_CP122617:c4386395-4386222 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT226736 WP_000729704.1 NZ_CP088131:3742072-3742332 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT226736 NZ_CP122617:c4386719-4386423 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References