Detailed information of TA system TA226735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3735883..3736577
• Replicon: chromosome
• Accession: NZ_CP088131
• Organism: Escherichia coli strain 81

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: LRM32_RS18340
• Protein ID: WP_001263493.1
• Coordinates: 3735883..3736281 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: LRM32_RS18345
• Protein ID: WP_000554757.1
• Coordinates: 3736284..3736577 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3731543)	3731543..3731623	-	81	NuclAT_10	-	-
- (3731543)	3731543..3731623	-	81	NuclAT_10	-	-
- (3731543)	3731543..3731623	-	81	NuclAT_10	-	-
- (3731543)	3731543..3731623	-	81	NuclAT_10	-	-
LRM32_RS18310 (3730883)	3730883..3732127	-	1245	WP_000189541.1	esterase FrsA	-
LRM32_RS18315 (3732219)	3732219..3732677	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LRM32_RS18320 (3732938)	3732938..3734395	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
LRM32_RS18325 (3734452)	3734452..3734973	-	522	Protein_3600	peptide chain release factor H	-
LRM32_RS18330 (3734972)	3734972..3735175	-	204	Protein_3601	RtcB family protein	-
LRM32_RS18335 (3735421)	3735421..3735873	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
LRM32_RS18340 (3735883)	3735883..3736281	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LRM32_RS18345 (3736284)	3736284..3736577	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LRM32_RS18350 (3736629)	3736629..3737684	-	1056	WP_001226175.1	DNA polymerase IV	-
LRM32_RS18355 (3737755)	3737755..3738540	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
LRM32_RS18360 (3738512)	3738512..3740224	+	1713	Protein_3607	flagellar biosynthesis protein FlhA	-
LRM32_RS18365 (3740440)	3740440..3740937	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T226735 WP_001263493.1 NZ_CP088131:c3736281-3735883 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T226735 NZ_CP122617:c4284057-4283707 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT226735 WP_000554757.1 NZ_CP088131:c3736577-3736284 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT226735 NZ_CP122617:c4284302-4284051 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6