Detailed information of TA system TA226718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 664706..665433
• Replicon: chromosome
• Accession: NZ_CP088131
• Organism: Escherichia coli strain 81

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1EX98
• Locus tag: LRM32_RS03295
• Protein ID: WP_000550192.1
• Coordinates: 664706..665020 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRM32_RS03300
• Protein ID: WP_000560266.1
• Coordinates: 665017..665433 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM32_RS03275 (660873)	660873..661859	-	987	WP_001301393.1	Gfo/Idh/MocA family oxidoreductase	-
LRM32_RS03280 (661938)	661938..662621	-	684	WP_001183046.1	vancomycin high temperature exclusion protein	-
LRM32_RS03285 (662698)	662698..663201	-	504	WP_001295542.1	M48 family metallopeptidase	-
LRM32_RS03290 (663286)	663286..664422	+	1137	WP_000018695.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
LRM32_RS03295 (664706)	664706..665020	+	315	WP_000550192.1	type II toxin-antitoxin system toxin HigB	Toxin
LRM32_RS03300 (665017)	665017..665433	+	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
LRM32_RS03305 (665478)	665478..667496	-	2019	WP_000121433.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
LRM32_RS03310 (667922)	667922..670273	-	2352	WP_000695486.1	alpha-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12063.08 Da
• Isoelectric Point: 10.0409

>T226718 WP_000550192.1 NZ_CP088131:664706-665020 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTPKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T226718 NZ_CP122617:c593147-592683 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT226718 WP_000560266.1 NZ_CP088131:665017-665433 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT226718 NZ_CP122617:c593482-593147 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: S1EX98