Detailed information of TA system TA226656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1230502..1231151
• Replicon: chromosome
• Accession: NZ_CP088119
• Organism: Enterobacter kobei strain 6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LRM31_RS05825
• Protein ID: WP_047027750.1
• Coordinates: 1230789..1231151 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2W0GCS7
• Locus tag: LRM31_RS05820
• Protein ID: WP_014882863.1
• Coordinates: 1230502..1230801 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM31_RS05810 (LRM31_05810)	1226927..1228900	+	1974	WP_047027749.1	PhoX family phosphatase	-
LRM31_RS05815 (LRM31_05815)	1229003..1230391	+	1389	WP_241449007.1	phenylalanine transporter	-
LRM31_RS05820 (LRM31_05820)	1230502..1230801	-	300	WP_014882863.1	helix-turn-helix transcriptional regulator	Antitoxin
LRM31_RS05825 (LRM31_05825)	1230789..1231151	-	363	WP_047027750.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRM31_RS05830 (LRM31_05830)	1231357..1232592	+	1236	WP_023332295.1	MFS transporter	-
LRM31_RS05835 (LRM31_05835)	1232608..1233633	+	1026	WP_045269819.1	sugar phosphate isomerase/epimerase	-
LRM31_RS05840 (LRM31_05840)	1233626..1234768	+	1143	WP_045269820.1	Gfo/Idh/MocA family oxidoreductase	-
LRM31_RS05845 (LRM31_05845)	1234844..1235815	+	972	WP_045269821.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-80)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13910.87 Da
• Isoelectric Point: 5.6482

>T226656 WP_047027750.1 NZ_CP088119:c1231151-1230789 [Enterobacter kobei]
MWNVETTDRFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKLFYNEMITLADVEYSRHLADKEAIWQR

Nucleotide

• Length: 363 bp

>T226656 NZ_CP122588:c4275478-4275164 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCTCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10860.61 Da
• Isoelectric Point: 9.8627

>AT226656 WP_014882863.1 NZ_CP088119:c1230801-1230502 [Enterobacter kobei]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT226656 NZ_CP122588:c4275714-4275481 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2W0GCS7