Detailed information of TA system TA226617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 61072..61597
• Replicon: plasmid pSU303_2
• Accession: NZ_CP088097
• Organism: Rhizobium leguminosarum bv. viciae strain SU303

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: RlegSU303_RS29205
• Protein ID: WP_231283944.1
• Coordinates: 61325..61597 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A7V8H8J0
• Locus tag: RlegSU303_RS29200
• Protein ID: WP_017957817.1
• Coordinates: 61072..61323 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RlegSU303_RS29190 (RlegSU303_29190)	57813..59156	-	1344	WP_026158362.1	adenylate/guanylate cyclase domain-containing protein	-
RlegSU303_RS29195 (RlegSU303_29195)	59599..60936	+	1338	WP_017957816.1	nucleotide sugar dehydrogenase	-
RlegSU303_RS29200 (RlegSU303_29200)	61072..61323	+	252	WP_017957817.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
RlegSU303_RS29205 (RlegSU303_29205)	61325..61597	+	273	WP_231283944.1	Txe/YoeB family addiction module toxin	Toxin
RlegSU303_RS29210 (RlegSU303_29210)	61652..62878	-	1227	WP_026158363.1	DUF3095 domain-containing protein	-
RlegSU303_RS29215 (RlegSU303_29215)	62952..63431	+	480	WP_017957820.1	class I SAM-dependent methyltransferase	-
RlegSU303_RS29220 (RlegSU303_29220)	63507..65297	-	1791	WP_017957821.1	ABC transporter ATP-binding protein	-
RlegSU303_RS29225 (RlegSU303_29225)	65642..66097	-	456	WP_017957822.1	Rrf2 family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..529550	529550

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10738.37 Da
• Isoelectric Point: 9.4534

>T226617 WP_231283944.1 NZ_CP088097:61325-61597 [Rhizobium leguminosarum bv. viciae]
MKLLWTPNSLEEYEYWQKNDQKMVEKINELLKDTKRSPFKGLGKPEPLKGDLSGFWSRRIHGEHRLVYCVTGKGANQQLE
IIQCRFHYEK

Nucleotide

• Length: 273 bp

>T226617 NZ_CP122578:c2621716-2620394 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCTCTCTCACTGCCGCTGCAGTATGGCAATATCACGG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAA
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTTACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTTTCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9287.55 Da
• Isoelectric Point: 5.6513

>AT226617 WP_017957817.1 NZ_CP088097:61072-61323 [Rhizobium leguminosarum bv. viciae]
MAHVRLTEFRQNIATYFDQVLSSRAPLLVTRQGSEAMVLVAEGEYESMQETLHLLSTPANSARLRESIGQLRKGQVIESD
PTL

Nucleotide

• Length: 252 bp

>AT226617 NZ_CP122578:c2621982-2621716 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7V8H8J0