Detailed information of TA system TA226611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 61620..62166
• Replicon: plasmid pWSM1455_2
• Accession: NZ_CP088092
• Organism: Rhizobium laguerreae strain WSM1455

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: RlegWSM1455_RS29410
• Protein ID: WP_132613658.1
• Coordinates: 61873..62166 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: RlegWSM1455_RS29405
• Protein ID: WP_168259268.1
• Coordinates: 61620..61871 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RlegWSM1455_RS29395 (RlegWSM1455_29395)	58267..59610	-	1344	WP_222342406.1	adenylate/guanylate cyclase domain-containing protein	-
RlegWSM1455_RS29400 (RlegWSM1455_29400)	60154..61491	+	1338	WP_168339675.1	nucleotide sugar dehydrogenase	-
RlegWSM1455_RS29405 (RlegWSM1455_29405)	61620..61871	+	252	WP_168259268.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
RlegWSM1455_RS29410 (RlegWSM1455_29410)	61873..62166	+	294	WP_132613658.1	Txe/YoeB family addiction module toxin	Toxin
RlegWSM1455_RS29415 (RlegWSM1455_29415)	62199..63425	-	1227	WP_077979945.1	DUF3095 domain-containing protein	-
RlegWSM1455_RS29420 (RlegWSM1455_29420)	63599..64078	+	480	WP_168259270.1	class I SAM-dependent methyltransferase	-
RlegWSM1455_RS29425 (RlegWSM1455_29425)	64146..65936	-	1791	WP_231285768.1	ABC transporter ATP-binding protein	-
RlegWSM1455_RS29430 (RlegWSM1455_29430)	66355..66810	-	456	WP_077979942.1	Rrf2 family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..521923	521923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-70)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11635.34 Da
• Isoelectric Point: 8.6999

>T226611 WP_132613658.1 NZ_CP088092:61873-62166 [Rhizobium laguerreae]
MKLQWTPNSWEEYEYWQKNDQKMVERIDELLKDTKRSPFKGLGKPEPLKGDLSGFWSRRILGEHRLVYCVTGKGSNQQLE
IIQCRFHYEKWVLAQCS

Nucleotide

• Length: 294 bp

>T226611 NZ_CP122576:158491-158823 [Klebsiella pneumoniae]
ATGGTAAAGCGTTATGTTCCAGATGCGGGCGATCTTATCTGGATTGATTTTGACCCGGTAGCCGGGCATGAACAGGGCGG
ACACCGCCCTGCTGTTGTTTTGAGTCCTTTTGCCTACAACAACAAAGTTGGCCTCCTTCTGTGTGTTCCATGCACGACGC
AAGTTAAGGGATACCCCTTTGAGGTCACGTTGTCCGGCAGCAAAGAAGGCGTAGCCCTGTCAGATCAGATAACCTGCGTT
GACTGGCGAGCCAGAAAAGTGACCCAAAAATCCACAGTAAGTAAGTCGGAGCTTGCAGAAATCCGGGCCAAAGCAAAGGC
GTTGATTGGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9273.53 Da
• Isoelectric Point: 5.6513

>AT226611 WP_168259268.1 NZ_CP088092:61620-61871 [Rhizobium laguerreae]
MAHVRLTEFRQNIATYFDQVLSSRAPLLVTRQGSEAMVLVAEGEYESMQETLHLLSTPANSARLRESLGQLRKGQVVESD
PTL

Nucleotide

• Length: 252 bp

>AT226611 NZ_CP122576:158246-158491 [Klebsiella pneumoniae]
ATGGCTCAGGTAATAGTAAAAAAATGGGGTAACAGCCCGTCAGTCCGGTTGCCAGTAGCAGTGATGAAAGCGGCATCGCT
AAATGTCGATGATACTGTCGATATTGAAGTAGAAGATGGTCGTATCATCATCGTCCCTGCAAGACCGAAAGAATATTCCC
TTGATGCGCTGCTGTCTGGTATTTCCTCCGATAATCTGCATGACAAAGTGGATTTTGGCCCTGCGAAAGGTAAGGAATTG
CTCTGA