Detailed information of TA system TA226578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 507188..507690
• Replicon: chromosome
• Accession: NZ_CP088019
• Organism: Pectobacterium carotovorum strain 25.1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A7W2E5L4
• Locus tag: LQF52_RS02260
• Protein ID: WP_039282578.1
• Coordinates: 507188..507442 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LQF52_RS02265
• Protein ID: WP_039534821.1
• Coordinates: 507439..507690 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQF52_RS02240 (LQF52_02240)	502341..503603	-	1263	WP_010296200.1	L-rhamnose isomerase	-
LQF52_RS02245 (LQF52_02245)	503600..505090	-	1491	WP_039534816.1	rhamnulokinase	-
LQF52_RS02250 (LQF52_02250)	505427..506266	+	840	WP_039546925.1	HTH-type transcriptional activator RhaS	-
LQF52_RS02255 (LQF52_02255)	506291..507145	+	855	WP_039546926.1	helix-turn-helix domain-containing protein	-
LQF52_RS02260 (LQF52_02260)	507188..507442	-	255	WP_039282578.1	Txe/YoeB family addiction module toxin	Toxin
LQF52_RS02265 (LQF52_02265)	507439..507690	-	252	WP_039534821.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LQF52_RS02270 (LQF52_02270)	507766..509472	-	1707	WP_039534824.1	ABC transporter ATP-binding protein	-
LQF52_RS02275 (LQF52_02275)	509469..510335	-	867	WP_010285293.1	ABC transporter permease	-
LQF52_RS02280 (LQF52_02280)	510328..511287	-	960	WP_039470517.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10229.79 Da
• Isoelectric Point: 8.0353

>T226578 WP_039282578.1 NZ_CP088019:c507442-507188 [Pectobacterium carotovorum]
VKLIWSEEAWEDYLYWQDIDKRMVKKINELIKETRRTPFEGKGKPEPLKHNLAGFWSRRITEEHRLVYAITDDAMMIAAC
RYHY

Nucleotide

• Length: 255 bp

>T226578 NZ_CP122518:64346-64744 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9366.70 Da
• Isoelectric Point: 5.1247

>AT226578 WP_039534821.1 NZ_CP088019:c507690-507439 [Pectobacterium carotovorum]
MRTISYSEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANAKRLMNSIESLKAGNGKERD
IIE

Nucleotide

• Length: 252 bp

>AT226578 NZ_CP122518:64119-64346 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A7W2E5L4