Detailed information of TA system TA022653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 612864..613663
• Replicon: chromosome
• Accession: NC_010468
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: ECOLC_RS03025
• Protein ID: WP_000347251.1
• Coordinates: 612864..613328 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: ECOLC_RS03030
• Protein ID: WP_012304834.1
• Coordinates: 613328..613663 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECOLC_RS02995	607865..608299	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
ECOLC_RS03000	608317..609195	-	879	WP_012304833.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ECOLC_RS03005	609185..609964	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ECOLC_RS03010	609975..610448	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ECOLC_RS03015	610471..611751	-	1281	WP_000681905.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ECOLC_RS03020	612000..612809	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
ECOLC_RS03025	612864..613328	-	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ECOLC_RS03030	613328..613663	-	336	WP_012304834.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ECOLC_RS03035	613812..615383	-	1572	WP_001273760.1	galactarate dehydratase	-
ECOLC_RS03045	615758..617092	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
ECOLC_RS03050	617108..617878	+	771	WP_001058236.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T22653 WP_000347251.1 NC_010468:c613328-612864 [Escherichia coli ATCC 8739]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T22653 NC_010468:c613328-612864 [Escherichia coli ATCC 8739]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12344.95 Da
• Isoelectric Point: 4.6890

>AT22653 WP_012304834.1 NC_010468:c613663-613328 [Escherichia coli ATCC 8739]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT22653 NC_010468:c613663-613328 [Escherichia coli ATCC 8739]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCCGGCCTGGACAGCATTCATTACGAAATTCTCCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20