Detailed information of TA system TA226528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 115875..116478
• Replicon: chromosome
• Accession: NZ_CP087994
• Organism: Acetobacterium wieringae strain Y

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LNN31_RS00610
• Protein ID: WP_263993143.1
• Coordinates: 116227..116478 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LNN31_RS00605
• Protein ID: WP_084633667.1
• Coordinates: 115875..116234 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN31_RS00580 (LNN31_00580)	111315..111647	+	333	WP_228878016.1	phage head-tail connector protein	-
LNN31_RS00585 (LNN31_00585)	111667..112095	+	429	WP_228878013.1	terminase small subunit	-
LNN31_RS00590 (LNN31_00590)	112079..113326	+	1248	WP_228878011.1	PBSX family phage terminase large subunit	-
LNN31_RS00595 (LNN31_00595)	113339..114694	+	1356	WP_228878008.1	phage portal protein	-
LNN31_RS00600 (LNN31_00600)	114748..115533	+	786	WP_228878006.1	sigma-70 family RNA polymerase sigma factor	-
LNN31_RS00605 (LNN31_00605)	115875..116234	-	360	WP_084633667.1	helix-turn-helix domain-containing protein	Antitoxin
LNN31_RS00610 (LNN31_00610)	116227..116478	-	252	WP_263993143.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LNN31_RS00615 (LNN31_00615)	116676..117074	-	399	WP_228878004.1	hypothetical protein	-
LNN31_RS00620 (LNN31_00620)	117022..117392	-	371	Protein_118	KilA-N domain-containing protein	-
LNN31_RS00625 (LNN31_00625)	117491..117916	-	426	WP_228878002.1	DUF3788 domain-containing protein	-
LNN31_RS00630 (LNN31_00630)	118122..118502	+	381	Protein_120	DUF1801 domain-containing protein	-
LNN31_RS00635 (LNN31_00635)	118695..119603	+	909	WP_228877999.1	hypothetical protein	-
LNN31_RS00640 (LNN31_00640)	119699..120349	+	651	WP_070371725.1	SOS response-associated peptidase	-
LNN31_RS00645 (LNN31_00645)	120482..120682	-	201	WP_070371724.1	PTS ascorbate transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	96772..124639	27867

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (3-72)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-88)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9910.42 Da
• Isoelectric Point: 9.4005

>T226528 WP_263993143.1 NZ_CP087994:c116478-116227 [Acetobacterium wieringae]
INDYIQMLSKYGTKIGEPYIKHLEGEIWEIRPLRNRIFFVAWVDGSYVLLHQFIKKSQKTPKREIEQAKRELAFLKESGE
NNE

Nucleotide

• Length: 252 bp

>T226528 NZ_CP122507:1804293-1804544 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13424.87 Da
• Isoelectric Point: 10.7792

>AT226528 WP_084633667.1 NZ_CP087994:c116234-115875 [Acetobacterium wieringae]
MNNKAIGSSWTDVRKEIFTPEEILESDLRVALMGEIIKARHDKGISQKKLEELSGVRQPVIARMERGSTDPKLTTVLKIL
EPLGMTLTIVPIKEKRMRLKPPFELNLSKNGGKHPIKRG

Nucleotide

• Length: 360 bp

>AT226528 NZ_CP122507:1804541-1804795 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA