Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4042870..4043467 Replicon chromosome
Accession NZ_CP087978
Organism Klebsiella variicola subsp. variicola strain ZH07

Toxin (Protein)


Gene name higB Uniprot ID A0A0B7GEF2
Locus tag LPW14_RS19655 Protein ID WP_012542526.1
Coordinates 4043150..4043467 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LPW14_RS19650 Protein ID WP_200790704.1
Coordinates 4042870..4043157 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPW14_RS19620 (LPW14_19620) 4038780..4039028 + 249 WP_008805539.1 DUF1158 domain-containing protein -
LPW14_RS19625 (LPW14_19625) 4039045..4039386 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LPW14_RS19630 (LPW14_19630) 4039417..4040532 - 1116 WP_162499993.1 MBL fold metallo-hydrolase -
LPW14_RS19635 (LPW14_19635) 4040712..4041293 + 582 WP_012968754.1 TetR/AcrR family transcriptional regulator -
LPW14_RS19640 (LPW14_19640) 4041293..4041661 + 369 WP_008805536.1 MmcQ/YjbR family DNA-binding protein -
LPW14_RS19645 (LPW14_19645) 4041781..4042434 + 654 WP_012968755.1 oxygen-insensitive NAD(P)H nitroreductase -
LPW14_RS19650 (LPW14_19650) 4042870..4043157 - 288 WP_200790704.1 helix-turn-helix transcriptional regulator Antitoxin
LPW14_RS19655 (LPW14_19655) 4043150..4043467 - 318 WP_012542526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LPW14_RS19660 (LPW14_19660) 4043652..4044695 - 1044 WP_230648490.1 DUF2157 domain-containing protein -
LPW14_RS19665 (LPW14_19665) 4045229..4046095 - 867 WP_022065427.1 helix-turn-helix transcriptional regulator -
LPW14_RS19670 (LPW14_19670) 4046204..4047631 + 1428 WP_023297179.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-89)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T226511 WP_012542526.1 NZ_CP087978:c4043467-4043150 [Klebsiella variicola subsp. variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T226511 NZ_CP122503:2744449-2744556 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10829.54 Da        Isoelectric Point: 10.2212

>AT226511 WP_200790704.1 NZ_CP087978:c4043157-4042870 [Klebsiella variicola subsp. variicola]
MSKKIIDWETLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Download         Length: 288 bp

>AT226511 NZ_CP122503:c2744402-2744336 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B7GEF2

Antitoxin

Source ID Structure

References