Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 365149..365795 Replicon chromosome
Accession NZ_CP087978
Organism Klebsiella variicola subsp. variicola strain ZH07

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag LPW14_RS01655 Protein ID WP_004174016.1
Coordinates 365149..365496 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag LPW14_RS01660 Protein ID WP_008806992.1
Coordinates 365496..365795 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPW14_RS01645 (LPW14_01645) 361036..362469 + 1434 WP_004202133.1 glycogen synthase GlgA -
LPW14_RS01650 (LPW14_01650) 362487..364934 + 2448 WP_008806990.1 glycogen phosphorylase -
LPW14_RS01655 (LPW14_01655) 365149..365496 + 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LPW14_RS01660 (LPW14_01660) 365496..365795 + 300 WP_008806992.1 XRE family transcriptional regulator Antitoxin
LPW14_RS01665 (LPW14_01665) 365858..367366 - 1509 WP_004202136.1 glycerol-3-phosphate dehydrogenase -
LPW14_RS01670 (LPW14_01670) 367570..367899 + 330 WP_032755936.1 thiosulfate sulfurtransferase GlpE -
LPW14_RS01675 (LPW14_01675) 367950..368780 + 831 WP_008806994.1 rhomboid family intramembrane serine protease GlpG -
LPW14_RS01680 (LPW14_01680) 368830..369588 + 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T226498 WP_004174016.1 NZ_CP087978:365149-365496 [Klebsiella variicola subsp. variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T226498 NZ_CP122501:c55632-55480 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT226498 WP_008806992.1 NZ_CP087978:365496-365795 [Klebsiella variicola subsp. variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT226498 NZ_CP122501:55684-55744 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References