Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 127449..128032 Replicon chromosome
Accession NZ_CP087977
Organism Janibacter limosus strain P1(28)-3

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag LP422_RS00815 Protein ID WP_257908997.1
Coordinates 127754..128032 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag LP422_RS00810 Protein ID WP_257908995.1
Coordinates 127449..127733 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LP422_RS00785 (LP422_00765) 122773..123930 - 1158 WP_257908987.1 LLM class flavin-dependent oxidoreductase -
LP422_RS00790 (LP422_00770) 123994..124832 - 839 Protein_155 SMP-30/gluconolactonase/LRE family protein -
LP422_RS00795 (LP422_00775) 124850..125839 - 990 WP_257908989.1 5,10-methylenetetrahydrofolate reductase -
LP422_RS00800 (LP422_00780) 125963..126721 + 759 WP_257908991.1 SDR family NAD(P)-dependent oxidoreductase -
LP422_RS00805 (LP422_00785) 126924..127391 - 468 WP_257908993.1 YbaK/EbsC family protein -
LP422_RS00810 (LP422_00790) 127449..127733 - 285 WP_257908995.1 HigA family addiction module antitoxin Antitoxin
LP422_RS00815 (LP422_00795) 127754..128032 - 279 WP_257908997.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LP422_RS00820 128215..128304 + 90 Protein_161 DUF4395 domain-containing protein -
LP422_RS00825 128408..128641 + 234 WP_257911111.1 DUF4395 domain-containing protein -
LP422_RS00830 128822..129244 - 423 WP_257908999.1 enoyl-CoA hydratase/isomerase family protein -
LP422_RS00835 (LP422_00810) 129570..130749 - 1180 Protein_164 thiolase family protein -
LP422_RS00840 (LP422_00815) 130826..131140 + 315 WP_257909001.1 hypothetical protein -
LP422_RS00845 (LP422_00820) 131137..131983 + 847 Protein_166 SDR family oxidoreductase -
LP422_RS00850 (LP422_00825) 131931..132524 - 594 WP_257909003.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(2-91)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10576.08 Da        Isoelectric Point: 9.9896

>T226494 WP_257908997.1 NZ_CP087977:c128032-127754 [Janibacter limosus]
VIVSFRHKGLERLYRTGTKKGVQAAHVPKLLRILSAPDVAQGPADLEIPSFRTHRLKGDLTGHHSIWVNGNWRITFRFIE
SDVELVDYQDYH

Download         Length: 279 bp

>T226494 NZ_CP122499:c4281522-4281349 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 9807.29 Da        Isoelectric Point: 9.0576

>AT226494 WP_257908995.1 NZ_CP087977:c127733-127449 [Janibacter limosus]
MMKNPVHPGEILREDVLADLGLTVSEAANRLGVSRVSLSRVLHGHAGISSTLAIRLEAAGVGTASSWLAMQSAHDLSVER
AAGIPKVQRLTSVA

Download         Length: 285 bp

>AT226494 NZ_CP122499:c4281846-4281550 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References