Detailed information of TA system TA226477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 964070..965510
• Replicon: chromosome
• Accession: NZ_CP087952
• Organism: Desulfosarcina sp. BuS5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BuS5_RS04720
• Protein ID: WP_027354943.1
• Coordinates: 964070..964408 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BuS5_RS04725
• Protein ID: WP_035266443.1
• Coordinates: 964401..965510 (+)
• Length: 370 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BuS5_RS04690 (BuS5_00968)	959199..960164	-	966	WP_157487501.1	DUF2971 domain-containing protein	-
BuS5_RS04695 (BuS5_00969)	960254..960958	-	705	WP_274428046.1	hypothetical protein	-
BuS5_RS04700 (BuS5_00970)	960951..961199	-	249	WP_027355009.1	hypothetical protein	-
BuS5_RS04705 (BuS5_00971)	961360..962277	-	918	WP_274428047.1	hypothetical protein	-
BuS5_RS04710 (BuS5_00972)	962391..963545	+	1155	WP_036019371.1	IS4 family transposase	-
BuS5_RS04715	963589..963711	+	123	WP_274428048.1	hypothetical protein	-
BuS5_RS04720 (BuS5_00973)	964070..964408	+	339	WP_027354943.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BuS5_RS04725 (BuS5_00974)	964401..965510	+	1110	WP_035266443.1	HigA family addiction module antitoxin	Antitoxin
BuS5_RS04730 (BuS5_00975)	965930..966994	+	1065	WP_027354941.1	hypothetical protein	-
BuS5_RS04735 (BuS5_00976)	966991..967278	+	288	WP_035266442.1	hypothetical protein	-
BuS5_RS04740 (BuS5_00978)	967704..967943	+	240	WP_027354939.1	MazF family transcriptional regulator	-
BuS5_RS04745 (BuS5_00979)	967943..968281	+	339	WP_027354938.1	endoribonuclease MazF	-
BuS5_RS04750 (BuS5_00980)	969345..969650	+	306	WP_027354937.1	nucleotidyltransferase domain-containing protein	-
BuS5_RS04755 (BuS5_00981)	969643..970068	+	426	WP_051375151.1	DUF86 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-80)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-73)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13137.34 Da
• Isoelectric Point: 10.5225

>T226477 WP_027354943.1 NZ_CP087952:964070-964408 [Desulfosarcina sp. BuS5]
MHIIFKTKRLEKELNFSKKLIKKYGSKTTRFIMRRMKVLAAANSLADVPHQPPERCHQLSGGRDEQFAVVIRDQWRLIFV
PNHETIPRKRDGGIELSQITAIEIIWIGDYHE

Nucleotide

• Length: 339 bp

>T226477 NZ_CP122498:36492-36773 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 370 a.a.
• Molecular weight: 41890.34 Da
• Isoelectric Point: 10.0916

>AT226477 WP_035266443.1 NZ_CP087952:964401-965510 [Desulfosarcina sp. BuS5]
MNNIETNAYQPDYTIHPGEILEETLEARGIKKTALAERCGITLKTVSQIINGKASISPSIAIRLERAIGVSASLWSNLNT
DYELFVAREKDHASLYKQKQWVKKFPLQQLSNLGILPRKKNLTENLSNLLSFFGVSTISAWNNFYLAPNVAYRRSVAYKS
QPESIATWLRIGELMSESVECAPFNKKKFKVALSEIRKVTNEPVEIFQKIMIESCRKSGVSLVFVPELSKTHVSGATKWL
NTNKAMIILSLRHKMDDHFWFNFFHEAGHILLHGKTEVFIEANNHIEKSEKEVEADVFASNILISKSNYKKLTSLTKFSR
QSISAFAKELDLSPGIIVGRLQHDGLIPYSWLNKLKRKFAFSAQQSHTL

Nucleotide

• Length: 1110 bp

>AT226477 NZ_CP122498:36251-36502 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA