Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 911757..912325 Replicon chromosome
Accession NZ_CP087952
Organism Desulfosarcina sp. BuS5

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag BuS5_RS04415 Protein ID WP_027355190.1
Coordinates 911757..912035 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag BuS5_RS04420 Protein ID WP_027355191.1
Coordinates 912035..912325 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BuS5_RS04390 (BuS5_00900) 906860..907291 + 432 WP_274428032.1 hypothetical protein -
BuS5_RS04395 907314..908432 + 1119 Protein_869 IS3 family transposase -
BuS5_RS04400 (BuS5_00904) 909135..910290 + 1156 Protein_870 IS4 family transposase -
BuS5_RS04405 (BuS5_00906) 910600..910866 + 267 WP_274428033.1 reverse transcriptase domain-containing protein -
BuS5_RS04410 (BuS5_00907) 910905..911213 + 309 WP_274427764.1 group II intron maturase-specific domain-containing protein -
BuS5_RS04415 (BuS5_00908) 911757..912035 + 279 WP_027355190.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BuS5_RS04420 (BuS5_00909) 912035..912325 + 291 WP_027355191.1 HigA family addiction module antitoxin Antitoxin
BuS5_RS04425 (BuS5_00910) 912468..912701 - 234 WP_274428034.1 hypothetical protein -
BuS5_RS04430 (BuS5_00911) 912923..913534 - 612 WP_274428035.1 hypothetical protein -
BuS5_RS04435 (BuS5_00912) 913868..915020 + 1153 Protein_877 IS4 family transposase -
BuS5_RS04440 (BuS5_00915) 915047..916465 + 1419 WP_255342841.1 N-6 DNA methylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 908217..908432 215
- flank IS/Tn - - 914596..914784 188


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-66)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10851.61 Da        Isoelectric Point: 10.4176

>T226476 WP_027355190.1 NZ_CP087952:911757-912035 [Desulfosarcina sp. BuS5]
MIKTFKHKGLRKFFENGNLAGIKPGHRQKLRLRLIALDTATCIEDMNIPGWRLHSLKGDHKGLWAIDVNRNWRIVFKFKD
GNAYIVDYEDYH

Download         Length: 279 bp

>T226476 NZ_CP122497:c37473-37096 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTACTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGTC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10610.41 Da        Isoelectric Point: 8.9642

>AT226476 WP_027355191.1 NZ_CP087952:912035-912325 [Desulfosarcina sp. BuS5]
MAMYNPPHPGEFIKDVYLEPLGVTPRGVALRLKVAPSTFSRLIKGQSSISPGMALRLSKCLGRSPESWLLMQDSYDLWKA
KQNVDLSDIEPLAIPA

Download         Length: 291 bp

>AT226476 NZ_CP122497:c37694-37473 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References