Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 749207..749733 Replicon chromosome
Accession NZ_CP087952
Organism Desulfosarcina sp. BuS5

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag BuS5_RS03540 Protein ID WP_232223022.1
Coordinates 749207..749425 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag BuS5_RS03545 Protein ID WP_027353380.1
Coordinates 749434..749733 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BuS5_RS03525 (BuS5_00717) 744521..746230 + 1710 WP_027353377.1 GspE/PulE family protein -
BuS5_RS03530 (BuS5_00718) 746376..747596 + 1221 WP_027353378.1 type II secretion system F family protein -
BuS5_RS03535 (BuS5_00719) 747616..748950 + 1335 WP_027353379.1 sigma-54 dependent transcriptional regulator -
BuS5_RS03540 (BuS5_00720) 749207..749425 + 219 WP_232223022.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BuS5_RS03545 (BuS5_00721) 749434..749733 + 300 WP_027353380.1 HigA family addiction module antitoxin Antitoxin
BuS5_RS03550 (BuS5_00722) 750085..750534 + 450 WP_084445744.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
BuS5_RS03555 (BuS5_00723) 750757..751035 + 279 WP_035264737.1 nucleotidyltransferase family protein -
BuS5_RS03560 (BuS5_00724) 751032..751367 + 336 WP_027353382.1 DUF86 domain-containing protein -
BuS5_RS03565 (BuS5_00725) 751373..751891 + 519 WP_027353383.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
BuS5_RS03570 (BuS5_00726) 751888..752334 + 447 WP_027353384.1 hypothetical protein -
BuS5_RS03575 (BuS5_00727) 752353..752658 + 306 WP_027353385.1 DUF4160 domain-containing protein -
BuS5_RS03580 (BuS5_00728) 752655..752918 + 264 WP_027353386.1 DUF2442 domain-containing protein -
BuS5_RS03585 (BuS5_00729) 753003..753203 + 201 WP_027353387.1 addiction module protein -
BuS5_RS03590 (BuS5_00731) 753637..754152 + 516 WP_198012203.1 type II secretion system protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(8-72)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-68)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8436.71 Da        Isoelectric Point: 9.8751

>T226474 WP_232223022.1 NZ_CP087952:749207-749425 [Desulfosarcina sp. BuS5]
MPNDIQRLAECKLVMLHNAGGINDLRVPPANRLEALRGTRKGRHSIRINDQWRICFKWRADGVYHVEITDYH

Download         Length: 219 bp

>T226474 NZ_CP122496:c4106667-4106494 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCATA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11240.29 Da        Isoelectric Point: 10.1736

>AT226474 WP_027353380.1 NZ_CP087952:749434-749733 [Desulfosarcina sp. BuS5]
MRDFPPVHPGEILLEEFLKPMGISQYRLAKDIGVAAMRINKIVRGKRGISADTALRLARYFGMSVEFWTGIQTHYETEMA
KMVLAGRLEKEVKIFAQAV

Download         Length: 300 bp

>AT226474 NZ_CP122496:c4106991-4106695 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References