Detailed information of TA system TA226454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3914515..3915117
• Replicon: chromosome
• Accession: NZ_CP087880
• Organism: Pseudocitrobacter corydidari isolate G163CM

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G163CM_RS18135
• Protein ID: WP_041686546.1
• Coordinates: 3914515..3914826 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G163CM_RS18140
• Protein ID: WP_015966547.1
• Coordinates: 3914827..3915117 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G163CM_RS18100 (3909910)	3909910..3910509	+	600	WP_015966555.1	glucose-1-phosphatase	-
G163CM_RS18105 (3910503)	3910503..3911369	+	867	WP_015966554.1	virulence factor BrkB family protein	-
G163CM_RS18110 (3911366)	3911366..3911803	+	438	WP_231825847.1	D-aminoacyl-tRNA deacylase	-
G163CM_RS18115 (3911848)	3911848..3912789	+	942	WP_231825848.1	fatty acid biosynthesis protein FabY	-
G163CM_RS18120 (3912790)	3912790..3913080	-	291	WP_231825849.1	type II toxin-antitoxin system RelE/ParE family toxin	-
G163CM_RS18125 (3913073)	3913073..3913315	-	243	WP_113858287.1	type II toxin-antitoxin system ParD family antitoxin	-
G163CM_RS18130 (3913377)	3913377..3914285	-	909	WP_231825850.1	alpha/beta hydrolase	-
G163CM_RS18135 (3914515)	3914515..3914826	+	312	WP_041686546.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G163CM_RS18140 (3914827)	3914827..3915117	+	291	WP_015966547.1	NadS family protein	Antitoxin
G163CM_RS18145 (3915631)	3915631..3915873	+	243	WP_015966546.1	CopG family transcriptional regulator	-
G163CM_RS18150 (3915873)	3915873..3916247	+	375	WP_231825851.1	type II toxin-antitoxin system VapC family toxin	-
G163CM_RS18155 (3916283)	3916283..3917212	-	930	WP_015966544.1	formate dehydrogenase accessory protein FdhE	-
G163CM_RS18160 (3917209)	3917209..3917844	-	636	WP_015966543.1	formate dehydrogenase cytochrome b556 subunit	-
G163CM_RS18165 (3917841)	3917841..3918743	-	903	WP_149464253.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12033.93 Da
• Isoelectric Point: 8.3730

>T226454 WP_041686546.1 NZ_CP087880:3914515-3914826 [Pseudocitrobacter corydidari]
MIFIETDFFTEDVQKLLNDDEYGRLQIFLTLNPLNGDVIPETGGLRKIRWVAEGTGKRGGVRVIYYYRASESEIRFLLIY
KKGIKDDLTAQEKAMLRMLNARW

Nucleotide

• Length: 312 bp

>T226454 NZ_CP122493:c16060-15644 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10733.38 Da
• Isoelectric Point: 11.0117

>AT226454 WP_015966547.1 NZ_CP087880:3914827-3915117 [Pseudocitrobacter corydidari]
MDKALFERLAQSMAQMNEIVAGERKPSRVFHIDALKIKEIRHASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIQALSR

Nucleotide

• Length: 291 bp

>AT226454 NZ_CP122493:c16287-16057 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA