Detailed information of TA system TA226448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2855553..2856247
• Replicon: chromosome
• Accession: NZ_CP087880
• Organism: Pseudocitrobacter corydidari isolate G163CM

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: G163CM_RS13340
• Protein ID: WP_231825301.1
• Coordinates: 2855553..2855951 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: G163CM_RS13345
• Protein ID: WP_015965767.1
• Coordinates: 2855954..2856247 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G163CM_RS13315 (2851076)	2851076..2851477	-	402	WP_231825297.1	sigma factor-binding protein Crl	-
G163CM_RS13320 (2851535)	2851535..2852779	-	1245	WP_231825298.1	esterase FrsA	-
G163CM_RS13325 (2852886)	2852886..2853344	-	459	WP_015965764.1	xanthine phosphoribosyltransferase	-
G163CM_RS13330 (2853598)	2853598..2855055	+	1458	WP_231825299.1	cytosol nonspecific dipeptidase	-
G163CM_RS13335 (2855094)	2855094..2855537	-	444	WP_231825300.1	GNAT family N-acetyltransferase	-
G163CM_RS13340 (2855553)	2855553..2855951	-	399	WP_231825301.1	type II toxin-antitoxin system YafO family toxin	Toxin
G163CM_RS13345 (2855954)	2855954..2856247	-	294	WP_015965767.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G163CM_RS13350 (2856300)	2856300..2857355	-	1056	WP_231825302.1	DNA polymerase IV	-
G163CM_RS13355 (2857533)	2857533..2857748	-	216	WP_188016906.1	(Na+)-NQR maturation NqrM	-
G163CM_RS13360 (2857751)	2857751..2858974	-	1224	WP_015965770.1	NADH:ubiquinone reductase (Na(+)-transporting) subunit F	-
G163CM_RS13365 (2858987)	2858987..2859583	-	597	WP_015965771.1	NADH:ubiquinone reductase (Na(+)-transporting) subunit E	-
G163CM_RS13370 (2859587)	2859587..2860225	-	639	WP_015965772.1	NADH:ubiquinone reductase (Na(+)-transporting) subunit D	-
G163CM_RS13375 (2860218)	2860218..2861012	-	795	WP_231825303.1	Na(+)-translocating NADH-quinone reductase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15312.65 Da
• Isoelectric Point: 9.7692

>T226448 WP_231825301.1 NZ_CP087880:c2855951-2855553 [Pseudocitrobacter corydidari]
MRVFKTKLIRLQLTEKELAALTADFISYKRDGILPDISGRDAAYDSPYTWPLVKSERVAHIHLASAEKPFPPQLRQFNRT
NDEAHLVYCQGAFDEQAWLLIAILKPQPHQQARDNNQMQKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T226448 NZ_CP122492:3824028-3824306 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11136.59 Da
• Isoelectric Point: 5.1570

>AT226448 WP_015965767.1 NZ_CP087880:c2856247-2855954 [Pseudocitrobacter corydidari]
MHRILAEKSVNITELRKNPARYFIDQPVAVLSNNKPAGYLLSASVFEALMDLLAEHQDVKNVTAQFRPSAARLEEMTRRA
ERYLNDVTDDDLNDFKE

Nucleotide

• Length: 294 bp

>AT226448 NZ_CP122492:3823765-3824025 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA