Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 4201516..4202216 Replicon chromosome
Accession NC_010322
Organism Pseudomonas putida GB-1

Toxin (Protein)


Gene name mqsR Uniprot ID B0KNY8
Locus tag PPUTGB1_RS19085 Protein ID WP_012273366.1
Coordinates 4201920..4202216 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID B0KNY7
Locus tag PPUTGB1_RS19080 Protein ID WP_012273365.1
Coordinates 4201516..4201917 (-) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPUTGB1_RS19060 4196653..4197474 - 822 WP_012273361.1 transporter substrate-binding domain-containing protein -
PPUTGB1_RS19065 4197551..4198480 - 930 WP_012273362.1 FAD-binding protein -
PPUTGB1_RS19070 4198481..4199230 - 750 WP_012273363.1 electron transfer flavoprotein subunit beta/FixA family protein -
PPUTGB1_RS19075 4199763..4201445 + 1683 WP_071984592.1 electron transfer flavoprotein-ubiquinone oxidoreductase -
PPUTGB1_RS19080 4201516..4201917 - 402 WP_012273365.1 type II toxin-antitoxin system MqsA family antitoxin Antitoxin
PPUTGB1_RS19085 4201920..4202216 - 297 WP_012273366.1 type II toxin-antitoxin system MqsR family toxin Toxin
PPUTGB1_RS19090 4202311..4203636 - 1326 WP_012273367.1 MFS transporter -
PPUTGB1_RS19095 4203709..4204188 - 480 WP_012273368.1 response regulator -
PPUTGB1_RS19100 4204358..4204834 - 477 WP_012273369.1 sigma-70 family RNA polymerase sigma factor -
PPUTGB1_RS19105 4205072..4206244 + 1173 WP_041166759.1 efflux RND transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(2-97)

Antitoxin

Antitoxin MqsA

(3-131)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11239.13 Da        Isoelectric Point: 9.4791

>T22637 WP_012273366.1 NC_010322:c4202216-4201920 [Pseudomonas putida GB-1]
MEKRTPHCPLERIRALLSAGRVNPTTASLRGAQALGMEFADMLQVIAGLERRDFYKSMSCYVNHRVWQDVYRPFTAKGYV
YLKLSVVDDVLIVSFKEL

Download         Length: 297 bp

>T22637 NC_010322:c4202216-4201920 [Pseudomonas putida GB-1]
ATGGAAAAGAGAACGCCTCATTGTCCGCTGGAGCGGATCAGGGCATTGCTGAGCGCTGGCCGCGTCAACCCTACAACGGC
CTCATTACGAGGCGCTCAGGCGTTAGGGATGGAGTTTGCGGACATGCTTCAGGTTATCGCGGGCCTGGAGCGCAGGGACT
TCTACAAAAGCATGTCTTGTTATGTCAATCATCGCGTTTGGCAAGATGTCTACAGGCCGTTTACGGCGAAAGGCTACGTG
TACCTGAAGCTGTCTGTGGTTGATGACGTTCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14551.58 Da        Isoelectric Point: 4.7589

>AT22637 WP_012273365.1 NC_010322:c4201917-4201516 [Pseudomonas putida GB-1]
MRCPICGGAELAPDTQDMPYRYKGVSTVIPGVAGDYCSACGEAVLGYDEAMRVSDLMSAFSRQVNAKDVDPDFIVSVRKK
FDLDQREAGEIFGGGVNAFSRYENGKTTPPVALVKLLKLLDRHPELFEEVRTA

Download         Length: 402 bp

>AT22637 NC_010322:c4201917-4201516 [Pseudomonas putida GB-1]
ATGAGATGCCCAATTTGCGGCGGTGCGGAGCTCGCGCCAGACACTCAGGACATGCCCTACCGCTACAAGGGGGTATCGAC
AGTGATCCCTGGTGTCGCGGGCGATTACTGCTCTGCCTGTGGTGAGGCAGTACTTGGCTACGATGAGGCTATGCGCGTCA
GCGATCTGATGTCTGCTTTCAGCCGCCAAGTCAATGCTAAGGATGTCGACCCTGATTTTATTGTTTCCGTGCGCAAGAAG
TTTGATCTGGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGAGGGGTCAATGCGTTCTCTCGATATGAAAACGGCAAGAC
CACGCCACCCGTGGCGCTGGTGAAACTGCTGAAACTGCTGGATCGCCACCCAGAGCTTTTCGAGGAAGTACGTACAGCCT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Y3UVL7

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y3UX09

References