Detailed information of TA system TA226355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2644325..2644931
• Replicon: chromosome
• Accession: NZ_CP087844
• Organism: Moraxella bovis strain SAM58105

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LP103_RS13535
• Protein ID: WP_228157738.1
• Coordinates: 2644325..2644660 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LP103_RS13540
• Protein ID: WP_078274722.1
• Coordinates: 2644650..2644931 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LP103_RS13490	2639386..2639520	+	135	WP_264676041.1	hypothetical protein	-
LP103_RS13495 (LP103_13370)	2639572..2639727	+	156	WP_158079680.1	hypothetical protein	-
LP103_RS13500 (LP103_13375)	2639722..2639997	-	276	WP_078274708.1	hypothetical protein	-
LP103_RS13505 (LP103_13380)	2640284..2640529	-	246	WP_078274709.1	hypothetical protein	-
LP103_RS13510 (LP103_13385)	2640652..2640981	-	330	WP_264676042.1	hypothetical protein	-
LP103_RS13515 (LP103_13390)	2641100..2641357	+	258	WP_112741693.1	hypothetical protein	-
LP103_RS13520 (LP103_13395)	2641850..2642725	+	876	WP_162860331.1	virulence factor TspB C-terminal domain-related protein	-
LP103_RS13525 (LP103_13400)	2642722..2643015	+	294	WP_078274720.1	DUF2523 family protein	-
LP103_RS13530 (LP103_13405)	2643101..2644261	+	1161	WP_078274721.1	zonular occludens toxin domain-containing protein	-
LP103_RS13535 (LP103_13410)	2644325..2644660	+	336	WP_228157738.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LP103_RS13540 (LP103_13415)	2644650..2644931	+	282	WP_078274722.1	NadS family protein	Antitoxin
LP103_RS13545 (LP103_13420)	2644981..2645805	+	825	WP_078274723.1	site-specific integrase	-
LP103_RS13550 (LP103_13425)	2645921..2646133	+	213	WP_078274724.1	hypothetical protein	-
LP103_RS13555 (LP103_13430)	2646173..2646895	-	723	WP_029103568.1	nitroreductase family protein	-
LP103_RS13560 (LP103_13435)	2647040..2648365	-	1326	WP_112741690.1	hemolysin family protein	-
LP103_RS13565 (LP103_13440)	2648475..2649233	-	759	WP_078274726.1	YciK family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-84)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12636.69 Da
• Isoelectric Point: 10.0972

>T226355 WP_228157738.1 NZ_CP087844:2644325-2644660 [Moraxella bovis]
MLHFAETSLFTKQIIDLMDDDEYRLLQTNLMKNPHQGDIVRGTGGVRKTRWSIDGKGKSGGSRIIYFFVDGAGIFFMLLA
YPKSKQTTLSADEKKEMLKLTTAIKEIYRAK

Nucleotide

• Length: 336 bp

>T226355 NZ_CP122451:1911077-1911331 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10425.98 Da
• Isoelectric Point: 10.0023

>AT226355 WP_078274722.1 NZ_CP087844:2644650-2644931 [Moraxella bovis]
VQNEFYDDLKASLTEALAIAKGEISPSRTFTYERPNIKDIRAKTGLSQTQFAQKLHISPKTLKNWEQGIRTPTGPAITLI
RLLDKNPDLISMA

Nucleotide

• Length: 282 bp

>AT226355 NZ_CP122451:1910829-1911080 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA