Detailed information of TA system TA022633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5365231..5365821
• Replicon: chromosome
• Accession: NC_010296
• Organism: Microcystis aeruginosa NIES-843

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B0JIU6
• Locus tag: MAE_RS25440
• Protein ID: WP_012268033.1
• Coordinates: 5365231..5365506 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MAE_RS25445
• Protein ID: WP_012268034.1
• Coordinates: 5365519..5365821 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAE_RS25410	5361059..5361499	-	441	WP_041804412.1	hypothetical protein	-
MAE_RS25415	5361661..5362674	-	1014	WP_012268030.1	aldo/keto reductase	-
MAE_RS25420	5362926..5363243	-	318	WP_002762345.1	hypothetical protein	-
MAE_RS25425	5363606..5364166	-	561	WP_012268031.1	5-formyltetrahydrofolate cyclo-ligase	-
MAE_RS25430	5364356..5364673	+	318	WP_002751734.1	glutaredoxin 3	-
MAE_RS25435	5364658..5365146	+	489	WP_012268032.1	tRNA adenosine(34) deaminase TadA	-
MAE_RS25440	5365231..5365506	+	276	WP_012268033.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MAE_RS25445	5365519..5365821	+	303	WP_012268034.1	HigA family addiction module antidote protein	Antitoxin
MAE_RS25450	5366050..5366310	+	261	Protein_5216	leucine-rich repeat domain-containing protein	-
MAE_RS25455	5366881..5367444	-	564	WP_012268037.1	DUF4334 domain-containing protein	-
MAE_RS25460	5367523..5367966	-	444	WP_012268038.1	recombinase	-
MAE_RS25465	5368107..5368925	-	819	WP_012268039.1	hypothetical protein	-
MAE_RS25470	5369291..5370454	+	1164	WP_012268040.1	aminotransferase class V-fold PLP-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-91)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10683.31 Da
• Isoelectric Point: 9.8052

>T22633 WP_012268033.1 NC_010296:5365231-5365506 [Microcystis aeruginosa NIES-843]
MIKSFRCKDTEKLYNKQFVKKFSGIERLAIKRLRILESANTLEALAGLPSNRLERLKGDRIGQYSIRINDQYRICFSWDD
AATNVEIVDYH

Nucleotide

• Length: 276 bp

>T22633 NC_010296:5365231-5365506 [Microcystis aeruginosa NIES-843]
ATGATTAAAAGCTTTCGATGTAAAGATACAGAAAAGCTATATAATAAGCAGTTTGTTAAAAAGTTTTCTGGGATTGAACG
ATTAGCGATCAAAAGATTGCGTATTTTAGAAAGTGCTAATACACTAGAAGCATTGGCAGGATTGCCGAGTAACCGCTTAG
AACGTTTAAAAGGAGATAGAATCGGTCAATACAGTATTCGCATCAATGACCAGTATCGTATTTGTTTCTCATGGGATGAT
GCAGCTACTAATGTTGAAATTGTTGATTATCATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11225.74 Da
• Isoelectric Point: 4.3690

>AT22633 WP_012268034.1 NC_010296:5365519-5365821 [Microcystis aeruginosa NIES-843]
MNNWKSPIHPGEILADELEEINLDVSQLATRINISENELEQILKGQGNITGDIALKLGRFFNTGAEIWMNLQKAYELDLA
REKLGNTLEKIIPYQSLSSG

Nucleotide

• Length: 303 bp

>AT22633 NC_010296:5365519-5365821 [Microcystis aeruginosa NIES-843]
ATGAATAATTGGAAAAGTCCTATCCACCCCGGTGAAATTTTAGCTGATGAATTAGAGGAAATTAATCTTGATGTTTCTCA
GCTTGCCACAAGAATTAATATCTCTGAGAATGAACTTGAGCAAATTCTCAAGGGACAAGGAAATATCACGGGAGATATTG
CTTTAAAATTAGGGCGTTTTTTTAATACAGGAGCAGAAATTTGGATGAATTTACAAAAAGCCTATGAGTTAGATCTAGCG
AGGGAAAAGTTAGGAAATACCCTCGAAAAAATTATTCCTTATCAATCCCTGTCTTCAGGATAA

Structures

Toxin

• AlphaFold DB: A0A841V676