Detailed information of TA system TA022631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3564174..3564786
• Replicon: chromosome
• Accession: NC_010296
• Organism: Microcystis aeruginosa NIES-843

Toxin (Protein)

• Gene name: graT
• Uniprot ID: L7EDC6
• Locus tag: MAE_RS16825
• Protein ID: WP_002733818.1
• Coordinates: 3564174..3564455 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: L7EDF0
• Locus tag: MAE_RS16830
• Protein ID: WP_002733868.1
• Coordinates: 3564472..3564786 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAE_RS16780	3559317..3559505	-	189	WP_002797077.1	hypothetical protein	-
MAE_RS16785	3559502..3559789	-	288	WP_002797078.1	cell division topological specificity factor MinE	-
MAE_RS16790	3559819..3560619	-	801	WP_002797079.1	septum site-determining protein MinD	-
MAE_RS16795	3560778..3561605	-	828	WP_012266644.1	septum site-determining protein MinC	-
MAE_RS16800	3561618..3561968	-	351	WP_002797081.1	hypothetical protein	-
MAE_RS16805	3562146..3562655	+	510	WP_002797082.1	hypothetical protein	-
MAE_RS16810	3562751..3562996	+	246	WP_197533159.1	hypothetical protein	-
MAE_RS16815	3563078..3563398	+	321	WP_002793380.1	hypothetical protein	-
MAE_RS16820	3563388..3563831	+	444	WP_002751101.1	type II toxin-antitoxin system YhaV family toxin	-
MAE_RS16825	3564174..3564455	+	282	WP_002733818.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MAE_RS16830	3564472..3564786	+	315	WP_002733868.1	HigA family addiction module antidote protein	Antitoxin
MAE_RS32465	3564954..3566110	-	1157	Protein_3460	IS630-like element ISMae25 family transposase	-
MAE_RS30110	3566138..3566986	-	849	WP_012264307.1	IS630-like element ISMae21 family transposase	-
MAE_RS16850	3567378..3568355	-	978	WP_012266647.1	hypothetical protein	-
MAE_RS33455	3568774..3568953	+	180	WP_080507009.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11148.73 Da
• Isoelectric Point: 10.2927

>T22631 WP_002733818.1 NC_010296:3564174-3564455 [Microcystis aeruginosa NIES-843]
MIKSFASKETEQVFKRKFSRKLPQDIQRNARRKLEVLDAAETLQDLYIPPSNRLEKLSGDRKNQHSIRINDQWRICFIWK
NGNAYNVEIVDYH

Nucleotide

• Length: 282 bp

>T22631 NC_010296:3564174-3564455 [Microcystis aeruginosa NIES-843]
ATGATTAAGAGTTTTGCATCTAAGGAGACAGAGCAGGTATTCAAACGTAAATTTTCACGAAAATTACCTCAAGATATTCA
ACGAAATGCTCGGAGAAAGCTAGAAGTTTTAGACGCGGCCGAAACTTTACAGGATTTATACATTCCTCCATCTAATCGGT
TGGAAAAACTCTCAGGAGATAGAAAAAATCAGCATAGTATCCGAATTAACGATCAGTGGCGGATCTGTTTTATTTGGAAA
AATGGCAACGCCTACAATGTAGAAATAGTCGATTATCATTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12332.33 Da
• Isoelectric Point: 4.8499

>AT22631 WP_002733868.1 NC_010296:3564472-3564786 [Microcystis aeruginosa NIES-843]
MTEKKLLAPIHPGEILLEEFLKPMEISQERLAEDLNVPIEQIKEIIEEKRGITADIALRLSRYFGMSERFWMNLQTRYDI
EVEKDRLRERLEKEVKVYAASQAL

Nucleotide

• Length: 315 bp

>AT22631 NC_010296:3564472-3564786 [Microcystis aeruginosa NIES-843]
ATGACTGAGAAAAAATTATTAGCTCCCATTCATCCTGGTGAGATTCTGTTAGAAGAATTTCTCAAACCGATGGAAATTAG
CCAAGAACGTTTGGCAGAAGATCTTAATGTACCAATAGAGCAAATCAAGGAAATTATCGAGGAAAAGCGAGGAATTACTG
CCGATATTGCCTTAAGGCTTTCTCGTTATTTTGGTATGTCTGAAAGGTTCTGGATGAATCTTCAAACAAGGTATGATATT
GAAGTAGAAAAAGATAGGCTCAGAGAACGTTTAGAGAAGGAAGTAAAAGTCTATGCTGCTTCACAAGCACTGTAG

Structures

Toxin

• AlphaFold DB: L7EDC6

Antitoxin

• AlphaFold DB: L7EDF0