Detailed information of TA system TA022627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1605875..1606481
• Replicon: chromosome
• Accession: NC_010296
• Organism: Microcystis aeruginosa NIES-843

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B0JWC2
• Locus tag: MAE_RS07810
• Protein ID: WP_012265096.1
• Coordinates: 1605875..1606156 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MAE_RS07815
• Protein ID: WP_002795680.1
• Coordinates: 1606167..1606481 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAE_RS07800	1601380..1602627	-	1248	WP_012265094.1	tryptophan synthase subunit beta	-
MAE_RS07805	1602764..1605334	-	2571	WP_012265095.1	DUF3536 domain-containing protein	-
MAE_RS07810	1605875..1606156	+	282	WP_012265096.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MAE_RS07815	1606167..1606481	+	315	WP_002795680.1	HigA family addiction module antidote protein	Antitoxin
MAE_RS34015	1606538..1606693	+	156	WP_012265097.1	hypothetical protein	-
MAE_RS28885	1606668..1606871	-	204	WP_197533173.1	Txe/YoeB family addiction module toxin	-
MAE_RS07820	1607104..1607358	-	255	WP_012265099.1	hypothetical protein	-
MAE_RS07825	1607589..1607927	-	339	WP_012265100.1	DUF2442 domain-containing protein	-
MAE_RS07830	1607881..1608099	-	219	WP_080507078.1	DUF4160 domain-containing protein	-
MAE_RS27660	1608269..1608697	-	429	WP_012265102.1	ricin-type beta-trefoil lectin domain protein	-
MAE_RS07840	1608836..1609120	-	285	WP_080506949.1	DUF1214 domain-containing protein	-
MAE_RS07845	1609272..1609493	-	222	WP_002795684.1	type II toxin-antitoxin system HicB family antitoxin	-
MAE_RS07850	1609496..1609723	-	228	WP_002744562.1	type II toxin-antitoxin system HicA family toxin	-
MAE_RS28890	1609841..1610029	+	189	WP_080507079.1	hypothetical protein	-
MAE_RS07855	1609991..1611003	-	1013	Protein_1609	adenine-specific methyltransferase EcoRI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (7-96)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10806.35 Da
• Isoelectric Point: 10.0074

>T22627 WP_012265096.1 NC_010296:1605875-1606156 [Microcystis aeruginosa NIES-843]
MIQSFRCKHTRLLFEGGYPPRFRGFKDTAIRKLTQLDAAQTLEFLRSPPGNQLGALKGDRKEQYSIRINSQFRLCFIWTN
NGPTDVEIVDYHS

Nucleotide

• Length: 282 bp

>T22627 NC_010296:1605875-1606156 [Microcystis aeruginosa NIES-843]
ATGATTCAATCTTTCCGATGCAAGCATACGCGATTACTTTTTGAAGGAGGATATCCGCCTCGTTTTCGCGGTTTCAAAGA
TACTGCTATTCGGAAGTTGACTCAATTAGATGCGGCTCAAACCTTAGAGTTTCTGCGCTCTCCACCAGGCAATCAACTTG
GAGCTTTAAAAGGCGATAGAAAAGAACAATATAGTATTCGGATTAATTCTCAGTTTCGATTATGTTTTATCTGGACAAAT
AATGGACCTACTGATGTTGAAATTGTTGATTATCACTCATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11802.65 Da
• Isoelectric Point: 9.8271

>AT22627 WP_002795680.1 NC_010296:1606167-1606481 [Microcystis aeruginosa NIES-843]
MNNRPINQMRPVHPGEILREDFLIPLNMSVNALALALKVPASRIHEIVKERRSVSADTAARLARYFGGDAASWLNLQVIY
DLKTLSNREEIIQQIQPRISEQIA

Nucleotide

• Length: 315 bp

>AT22627 NC_010296:1606167-1606481 [Microcystis aeruginosa NIES-843]
ATGAATAACCGTCCTATTAATCAAATGCGACCCGTTCACCCGGGAGAAATTCTGCGGGAAGATTTTTTGATTCCTCTAAA
TATGAGCGTGAATGCTTTAGCTTTAGCCTTGAAGGTTCCTGCATCTCGTATTCATGAAATTGTTAAAGAACGTCGTTCTG
TTTCGGCTGATACGGCAGCAAGATTAGCCCGCTATTTTGGCGGGGATGCTGCTTCCTGGCTCAATCTTCAAGTAATTTAT
GATCTGAAGACTTTATCTAACCGTGAGGAGATTATTCAGCAAATTCAGCCCCGTATTTCCGAACAAATTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A3G9JR44