Detailed information of TA system TA226090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 24594..25245
• Replicon: plasmid pAP2044-2
• Accession: NZ_CP087718
• Organism: Acinetobacter pittii strain AP2044

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9BC17
• Locus tag: LPS07_RS20440
• Protein ID: WP_004967307.1
• Coordinates: 24594..24950 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LPS07_RS20445
• Protein ID: WP_001140620.1
• Coordinates: 24943..25245 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPS07_RS20410 (LPS07_20410)	20107..20427	+	321	Protein_22	integrase core domain-containing protein	-
LPS07_RS20415 (LPS07_20415)	20562..21377	+	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
LPS07_RS20420 (LPS07_20420)	21464..21676	+	213	Protein_24	phosphoglucosamine mutase	-
LPS07_RS20425 (LPS07_20425)	21726..22658	+	933	WP_086374392.1	IS5 family transposase	-
LPS07_RS20430 (LPS07_20430)	22734..23537	+	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
LPS07_RS20435 (LPS07_20435)	23537..24373	+	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
LPS07_RS20440 (LPS07_20440)	24594..24950	+	357	WP_004967307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LPS07_RS20445 (LPS07_20445)	24943..25245	+	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
LPS07_RS20450 (LPS07_20450)	25327..25602	-	276	WP_004967304.1	hypothetical protein	-
LPS07_RS20455 (LPS07_20455)	25880..26104	-	225	WP_005155986.1	hypothetical protein	-
LPS07_RS20460 (LPS07_20460)	26256..26501	+	246	WP_057692601.1	hypothetical protein	-
LPS07_RS20465 (LPS07_20465)	26906..27400	+	495	WP_069120323.1	MobC family plasmid mobilization relaxosome protein	-
LPS07_RS20470 (LPS07_20470)	27390..29864	+	2475	WP_069120320.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / tet(39) / cmlB1 / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..43577	43577

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13601.62 Da
• Isoelectric Point: 6.4648

>T226090 WP_004967307.1 NZ_CP087718:24594-24950 [Acinetobacter pittii]
MWIVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIADKGSKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T226090 NZ_CP121833:c3589933-3588614 [Aeromonas veronii]
ATGGCCAATCTGGCTGTCTATATGAACGGCTATCGGGTAGGCACATTCACCAAAACTACCAGCGGCGCCCATCATTTTCA
GTACCATGAGTCATGGCTAGCACAACCGGGCAGCCGTCCGATCTCACTCTCCATGCCATTGCGCCATCAGACCTACCGGG
GCGATGAAGCCTATAACTTCTTCGACAACCTGCTGCCAGATAATACGGAGGTGAGAAACAGGGTTGTAGCACGCTATCAA
GCGGCATCAACCCAGCCCTTCGATCTGCTCAGTTGCATTGGCCAGGATAGCGTAGGGGCATTACAGCTGGTTACCGAAGG
GCACGATGTTCCAGATGTCAGGCGAATCGACTACAAGGCACTTTCCGATGACGAGCTCGAGCAGATCCTGACCAGCTACA
AATCAGGAATCCCTTTGGGGATGGTCAGGGAGCAAGAGGAGTTCCGGATCTCTATCGCCAGGGCGCAAGAAAAGACTGCA
CTGCTCTATCTCGATGGCCGTTGGTACTTGCCACTCAATGCAACGCCGACAACCCACATAATCAAACTCCCTATCGGCAA
GATTGAAAGCCACTCCTACTCGATAGACCTCTCCCAGAGCGTAGAAAACGAATATCTCTGCACTCTGATTGCCAAGGAGT
TTGGTCTACCCGTACCTCACTGCTTCATGATGCAGGTTGGCCAGGTCAAAGCACTGGCTGTGGAACGCTTCGACAGACGT
TACGCTGCCGATCGCAGTTGGATCATGCGCCTTCCCCAAGAAGATTTCTGTCAGGTATTGAATGTTCCGTCAGCACGGAA
ATACGAGAGCCATGGAGGACCAGGGATCACTGACATCATGAAAACCTTGCTGGGCTCTGCGACACCAGAACAGGATCGCT
ACCTGTTCATGAAATCTCAGGTCCTGTTCTGGCTGCTGGCGGCCACCGATGGCCATGCAAAGAACTTCTCGGTCTTTATT
GAACCGGAAGGGCGTTTTCGCCTGACCCCTTTTTACGACATCCTATCTGTCTATCCAGTGTTTGGCGGCCGAGGCCTCAA
CCGAAGGGACGCCAAGCTCGCTATGGGCCTAGCAAGCAGCAAGGGAAAGAAATACGCCATTGAGCAGATATTCCCGCGTC
ATTTCTTCCAAACGGCGAAAGCTGTGGGATTTGAGCGTTCTGCTATGGAAGAGATCCTGACAGAACTGGCCAACTCCGTT
GATGATGTTATTGAGCGTGTTACCCAGCAATTGCCAGCGGGCTTCCCCGATGCCATCAGCAGCACCATTCTAGAGGGCCT
AAAAGCCCGCTCAGCCAGGCTGATAAAGGGCTGGGATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT226090 WP_001140620.1 NZ_CP087718:24943-25245 [Acinetobacter pittii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT226090 NZ_CP121833:c3590187-3589933 [Aeromonas veronii]
ATGAAAGTAACCAGCGCCAAGCAGCTGAGCGCCTGTATCAAGGATGCCAGGCTTTCTCAAAAGCTCTCTCAAGGCAAAGT
GGCCGAGAAAGTAGGGATTAGGCAAGACACAGTCTCCAGCTTCGAGCTCAACCCTGAGTCCACCAAGCTGGAGACCCTGT
TCAAGATCCTCGCGGCACTCGAGTTGTCAGTGGATATCAAACCCCGCAATGAGTCATCCGAGAGTCCGGCATCGGGCTGG
AAGGAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7T4TW67