Detailed information of TA system TA226089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3811..4396
• Replicon: plasmid pAP2044-2
• Accession: NZ_CP087718
• Organism: Acinetobacter pittii strain AP2044

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: LPS07_RS20340
• Protein ID: WP_000897307.1
• Coordinates: 3811..4131 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LPS07_RS20345
• Protein ID: WP_000369781.1
• Coordinates: 4124..4396 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPS07_RS20305 (LPS07_20305)	482..679	-	198	Protein_1	IS30 family transposase	-
LPS07_RS20310 (LPS07_20310)	1232..1519	+	288	WP_000438825.1	BrnT family toxin	-
LPS07_RS20315 (LPS07_20315)	1506..1820	+	315	WP_230445356.1	BrnA antitoxin family protein	-
LPS07_RS20320 (LPS07_20320)	1909..2439	-	531	WP_000172759.1	hypothetical protein	-
LPS07_RS20325 (LPS07_20325)	2588..2890	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LPS07_RS20330 (LPS07_20330)	2891..3172	+	282	WP_000985609.1	putative addiction module antidote protein	-
LPS07_RS20335 (LPS07_20335)	3252..3671	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
LPS07_RS20340 (LPS07_20340)	3811..4131	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LPS07_RS20345 (LPS07_20345)	4124..4396	+	273	WP_000369781.1	NadS family protein	Antitoxin
LPS07_RS20350 (LPS07_20350)	4889..6364	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
LPS07_RS20355 (LPS07_20355)	6420..7304	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
LPS07_RS20360 (LPS07_20360)	7494..8105	-	612	WP_015060246.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / tet(39) / cmlB1 / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..43577	43577
	-	inside	IScluster/Tn	msr(E) / mph(E) / tet(39) / cmlB1	-	482..13216	12734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T226089 WP_000897307.1 NZ_CP087718:3811-4131 [Acinetobacter pittii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T226089 NZ_CP121817:c4324128-4322809 [Aeromonas veronii]
ATGGCCAATCTGGCTGTCTATATGAACGGCTATCGGGTAGGCACATTCACCAAAACTACCAGCGGCACCCATCATTTTCA
GTACCATGAGTCATGGCTAGCACAACCGGGCAGCCGTCCGATCTCACTCTCCATGCCATTGCGCCATCAGACCTACCGGG
GCGATGAAGCCTATAACTTCTTCGACAACCTGCTGCCAGATAATACGGAGGTGAGAAACAGGGTTGTAGCACGCTATCAA
GCGGCATCAACCCAGCCCTTCGATCTGCTCAGTTGCATTGGCCAGGATAGCGTAGGGGCATTACAGCTGGTTACCGAAGG
GCACGATGTTCCAGATGTCAGGCGAATCGACTACAAGGCACTTTCCGATGACGAGCTCGAGCAGATCCTGACCAGCTACA
AATCAGGAATCCCTTTGGGGATGGTCAGGGAGCAAGAGGAGTTCCGGATCTCTATCGCCGGGGCGCAAGAAAAGACTGCA
CTGCTCTATCTCGATGGCCGTTGGTACTTGCCACTCAATGCAACGCCGACAACCCACATAATCAAACTCCCTATCGGCAA
GATTGAAAGCCACTCCTACTCGATAGACCTCTCCCAGAGCGTAGAAAACGAATATCTCTGCACTCTGATTGCCAAGGAGT
TTGGTCTACCCGTACCTCACTGCTTCATGATGCAGGTTGGCCAGGTCAAAGCACTGGCTGTGGAACGCTTCGACAGACGT
TACGCTGCCGATCGCAGTTGGATCATGCGCCTTCCCCAAGAAGATTTCTGTCAGGTATTGAATGTTCCGTCAGCACGGAA
ATACGAGAACCATGGAGGACCGGGGATCACTGACATCATGAAAACCTTGCTGGGCTCTGCGACACCAGAACAGGATCGCT
ACCTATTCATGAAATCTCAGGTCCTGTTCTGGCTGCTGGCGGCCACCGATGGCCATGCAAAAAACTTCTCGGTCTTTATT
GAGCCGGAAGGGCGTTTTCGCCTGACCCCTTTTTACGACATCCTATCTGTCTATCCAGTATTTGGCGGCCGAGGCCTCAA
CCGAAGGGACGCCAAGCTCGCTATGGGCCTAGCAAGCAGCAAGGGAAAGAAGTACGCCATTGAGCAGATATTCCCGCGTC
ATTTCTTCCAAACGGCGAAAGCTGTGGGATTTGAGCGTTCTGCTATGGAAGAGATCCTGACAGAACTGGCCAACTCCGTT
GATGATGTTATTGAGCGCGTTACACAGCAATTGCCAGCAGGCTTCCCCGATACCATCAGCAGCACCATTCTAGAGGGCCT
AAAAGCCCGCTCAGCCAGGCTGATAAAGGGCTGGGATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT226089 WP_000369781.1 NZ_CP087718:4124-4396 [Acinetobacter pittii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT226089 NZ_CP121817:c4324382-4324128 [Aeromonas veronii]
ATGAAAGTAACCAGCGCCAAGCAGCTGAGCGCCTGTATCAAGGATGCCAGGCTTTCTCAAAAGCTCTCTCAAGGCAAAGT
GGCCGAGAAAGTAGGGATTAGGCAAGACACAGTCTCCAGCTTCGAGCTCAACCCTGAGTCCACCAAGCTGGAGACCCTGT
TCAAGATCCTCGCGGCACTCGAGTTGTCAGTGGATATCAAACCCCGCAATGAGTCATCCGAGAGTCCGGCATCGGGCTGG
AAGGAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3