Detailed information of TA system TA226073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3852345..3852942
• Replicon: chromosome
• Accession: NZ_CP087706
• Organism: Klebsiella pneumoniae strain YZH2487

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: LO768_RS19030
• Protein ID: WP_004893639.1
• Coordinates: 3852625..3852942 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: LO768_RS19025
• Protein ID: WP_004142561.1
• Coordinates: 3852345..3852632 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO768_RS18995 (3848563)	3848563..3848811	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
LO768_RS19000 (3848829)	3848829..3849170	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
LO768_RS19005 (3849201)	3849201..3850316	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
LO768_RS19010 (3850496)	3850496..3851066	+	571	Protein_3729	TetR/AcrR family transcriptional regulator	-
LO768_RS19015 (3851066)	3851066..3851434	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
LO768_RS19020 (3851554)	3851554..3852207	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
LO768_RS19025 (3852345)	3852345..3852632	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
LO768_RS19030 (3852625)	3852625..3852942	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO768_RS19035 (3853127)	3853127..3854170	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
LO768_RS19040 (3854840)	3854840..3855706	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
LO768_RS19045 (3855815)	3855815..3857242	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T226073 WP_004893639.1 NZ_CP087706:c3852942-3852625 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T226073 NZ_CP121769:c1424607-1424425 [Glaesserella parasuis]
GTGGATAGTGAAACGATGATAAAACTTCTCAAAAAGAACGGTTGGGAGCTAGATAGCATAGTCGGTAGCCACCACCACTT
TGAGAAGAAAAATCAAAAGGGAGCAGGCAAAGTGACTGTTCCCCACCCGAGAAAAGACTTAGGCTTTCTCGAAAAGAAAA
TCAGAAAACAAGCGGGGCTGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT226073 WP_004142561.1 NZ_CP087706:c3852632-3852345 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT226073 NZ_CP121769:c1424386-1423979 [Glaesserella parasuis]
ATGTTATACCCACTCATTTTTGAACAAGTTTCCGACGGCTATGTCGTTGCTATCCCAGATATTGACGGCTGTTATTCTGC
TGGAGATACCCTAGAAGAAACTTATAGCAACGTAAAACAAGCTATAGCACTTCATCTCGAATCTGTCGTCAAAGATGGGG
GAGAAATTCCAACGCCTACCGCTATCGAAAACCACAAAAACGACCCTGATTTAGACGGTCATAACGTATTTTTCGGTGTG
GTTGATGTCGATTTATCCCACCTAATGGGCAAAGCAGAGAAAATCAACATCACCCTCCCTAGCTATCTCATCAAACGCAT
TGATGATTTTGTTGCGGTTCACAAAGAGTATAAAAACCGTAGCAACTTCTTAGCTAAAATCGCAGCGGATAAAATCTTAA
CCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1