Detailed information of TA system TA226040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 38269..38801
• Replicon: chromosome
• Accession: NZ_CP087694
• Organism: Xylella taiwanensis strain PLS433

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Z9JHK5
• Locus tag: LPH46_RS00220
• Protein ID: WP_038272512.1
• Coordinates: 38269..38541 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: Z9JHR3
• Locus tag: LPH46_RS00225
• Protein ID: WP_038272513.1
• Coordinates: 38541..38801 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH46_RS00195 (LPH46_00195)	33651..34862	-	1212	WP_038272508.1	signal recognition particle-docking protein FtsY	-
LPH46_RS00200 (LPH46_00200)	35707..36180	+	474	WP_038272510.1	LEA type 2 family protein	-
LPH46_RS00205 (LPH46_00205)	36456..37070	+	615	WP_081755477.1	HNH endonuclease	-
LPH46_RS00210 (LPH46_00210)	37567..37830	+	264	WP_268746652.1	DUF3693 domain-containing protein	-
LPH46_RS00215 (LPH46_00215)	37889..38233	+	345	WP_038272511.1	DUF3653 domain-containing protein	-
LPH46_RS00220 (LPH46_00220)	38269..38541	-	273	WP_038272512.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
LPH46_RS00225 (LPH46_00225)	38541..38801	-	261	WP_038272513.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LPH46_RS00230 (LPH46_00230)	38890..40017	-	1128	WP_114867127.1	zonular occludens toxin domain-containing protein	-
LPH46_RS00235 (LPH46_00235)	40030..40374	-	345	WP_038273069.1	DUF2523 family protein	-
LPH46_RS00240 (LPH46_00240)	40377..41762	-	1386	WP_230428213.1	furin-like repeat-containing protein	-
LPH46_RS00245 (LPH46_00245)	41942..42178	-	237	WP_230428212.1	major capsid protein	-
LPH46_RS00250 (LPH46_00250)	42187..42393	-	207	WP_038273075.1	hypothetical protein	-
LPH46_RS00255 (LPH46_00255)	42396..42710	-	315	WP_230428211.1	hypothetical protein	-
LPH46_RS00260 (LPH46_00260)	42986..43573	-	588	WP_038273076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	35707..54142	18435

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-88)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10638.26 Da
• Isoelectric Point: 9.9110

>T226040 WP_038272512.1 NZ_CP087694:c38541-38269 [Xylella taiwanensis]
MRRPAYSSQFKRDVRVSQKRGKDIEKLKILITLLIEGSPLPAMYLDHPLKGQWRSFRDAHVEPDWLLIYKVDGDVVRFER
TGRHADLFDD

Nucleotide

• Length: 273 bp

>T226040 NZ_CP121698:c1359899-1359642 [Xanthomonas sacchari]
GTGACGCTGCAGTTCTCGGACAACGCCTGGGAGGATTATCTCTACTGGCAACAGGCCGACAAAAAGATGCTCAAGCGCAT
CAACGACCTGATCAAGGCCATTCAACGCGATCCCTTCCAGGGCATCGGCAAACCCGAACCGCTACGCCATGCACTTGCAG
GCTACTGGTCGCGACGCATCAACGACGAACACCGGATCGTCTACAAGGTCGAGCATGGCGTTCTGCTGATTGCACAAGTG
CGATATCACTACGTTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9440.91 Da
• Isoelectric Point: 4.8047

>AT226040 WP_038272513.1 NZ_CP087694:c38801-38541 [Xylella taiwanensis]
MAANALVRARVDEALKDEAASVLATMGLTVSDLIRITLTKVAREKMLPFEMHSPNRLTVETFVKSECGEDVHYAESADAM
FKDLGI

Nucleotide

• Length: 261 bp

>AT226040 NZ_CP121698:c1360147-1359896 [Xanthomonas sacchari]
ATGGACACCATCACCTATAGCGCCGCACGTGCCGCTCTCGCGGACACCATGGACCGCGTGGTCAACAACCACGAGCCGGT
GATCATCACACGCAGCCGCGAGCAAGCCGTTGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTATC
TGCTGCGCAGCCCGAAGAACGCGCAGCGCTTGCTGGAATCCATCGCCCAACTCGAATCCGGTCGCGGCAAGGCGCGGGAA
CTGAGCGAGTGA

Structures

Toxin

• AlphaFold DB: Z9JHK5

Antitoxin

• AlphaFold DB: Z9JHR3