Detailed information of TA system TA225953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5847705..5848300
• Replicon: chromosome
• Accession: NZ_CP087675
• Organism: Pseudomonas aeruginosa strain P93127

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: LO759_RS27670
• Protein ID: WP_003117425.1
• Coordinates: 5848022..5848300 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO759_RS27665
• Protein ID: WP_003113527.1
• Coordinates: 5847705..5848010 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO759_RS27630 (LO759_27630)	5842844..5843692	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
LO759_RS27640 (LO759_27640)	5843859..5844800	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
LO759_RS27645 (LO759_27645)	5844917..5845531	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
LO759_RS27650 (LO759_27650)	5845573..5846157	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
LO759_RS27655 (LO759_27655)	5846198..5847298	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
LO759_RS27665 (LO759_27665)	5847705..5848010	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
LO759_RS27670 (LO759_27670)	5848022..5848300	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO759_RS27675 (LO759_27675)	5848353..5848481	-	129	Protein_5483	integrase	-
LO759_RS27680 (LO759_27680)	5848629..5850857	+	2229	WP_003113525.1	TonB-dependent receptor	-
LO759_RS27685 (LO759_27685)	5850927..5851574	-	648	WP_003095021.1	carbonate dehydratase	-
LO759_RS27690 (LO759_27690)	5851636..5852874	-	1239	WP_003162396.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T225953 WP_003117425.1 NZ_CP087675:c5848300-5848022 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T225953 NZ_CP121613:c10126-9839 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT225953 WP_003113527.1 NZ_CP087675:c5848010-5847705 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT225953 NZ_CP121613:c9852-9538 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5