Detailed information of TA system TA225947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5874129..5874724
• Replicon: chromosome
• Accession: NZ_CP087674
• Organism: Pseudomonas aeruginosa strain P4970C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: LO758_RS27845
• Protein ID: WP_003117425.1
• Coordinates: 5874446..5874724 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO758_RS27840
• Protein ID: WP_003113527.1
• Coordinates: 5874129..5874434 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO758_RS27805 (LO758_27805)	5869268..5870116	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
LO758_RS27815 (LO758_27815)	5870283..5871224	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
LO758_RS27820 (LO758_27820)	5871341..5871955	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
LO758_RS27825 (LO758_27825)	5871997..5872581	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
LO758_RS27830 (LO758_27830)	5872622..5873722	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
LO758_RS27840 (LO758_27840)	5874129..5874434	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
LO758_RS27845 (LO758_27845)	5874446..5874724	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO758_RS27850 (LO758_27850)	5874777..5874905	-	129	Protein_5518	integrase	-
LO758_RS27855 (LO758_27855)	5875053..5877281	+	2229	WP_003113525.1	TonB-dependent receptor	-
LO758_RS27860 (LO758_27860)	5877351..5877998	-	648	WP_003095021.1	carbonate dehydratase	-
LO758_RS27865 (LO758_27865)	5878060..5879298	-	1239	WP_003162396.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T225947 WP_003117425.1 NZ_CP087674:c5874724-5874446 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T225947 NZ_CP121611:c5312-4992 [Acinetobacter baumannii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT225947 WP_003113527.1 NZ_CP087674:c5874434-5874129 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT225947 NZ_CP121611:c4999-4727 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5