Detailed information of TA system TA225941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5932132..5932727
• Replicon: chromosome
• Accession: NZ_CP087673
• Organism: Pseudomonas aeruginosa strain P96131

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LO755_RS28110
• Protein ID: WP_003113526.1
• Coordinates: 5932449..5932727 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO755_RS28105
• Protein ID: WP_003133769.1
• Coordinates: 5932132..5932437 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO755_RS28090 (LO755_28090)	5928854..5929717	-	864	WP_023098850.1	integrase domain-containing protein	-
LO755_RS28095 (LO755_28095)	5930318..5931460	-	1143	WP_023098851.1	STY4528 family pathogenicity island replication protein	-
LO755_RS28105 (LO755_28105)	5932132..5932437	-	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
LO755_RS28110 (LO755_28110)	5932449..5932727	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO755_RS28115 (LO755_28115)	5932780..5932908	-	129	Protein_5570	integrase	-
LO755_RS28120 (LO755_28120)	5933056..5935284	+	2229	WP_023104079.1	TonB-dependent receptor	-
LO755_RS28125 (LO755_28125)	5935355..5936002	-	648	WP_003095021.1	carbonate dehydratase	-
LO755_RS28130 (LO755_28130)	5936064..5937302	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T225941 WP_003113526.1 NZ_CP087673:c5932727-5932449 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T225941 NZ_CP121598:c2584967-2584785 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT225941 WP_003133769.1 NZ_CP087673:c5932437-5932132 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT225941 NZ_CP121598:c2584692-2584288 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Toxin

• AlphaFold DB: V6ALY3