Detailed information of TA system TA225924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3799852..3800449
• Replicon: chromosome
• Accession: NZ_CP087669
• Organism: Klebsiella pneumoniae strain KP53

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: LOM31_RS18800
• Protein ID: WP_004893639.1
• Coordinates: 3800132..3800449 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: LOM31_RS18795
• Protein ID: WP_004142561.1
• Coordinates: 3799852..3800139 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOM31_RS18765 (3796059)	3796059..3796307	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
LOM31_RS18770 (3796325)	3796325..3796666	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
LOM31_RS18775 (3796697)	3796697..3797812	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
LOM31_RS18780 (3797992)	3797992..3798573	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
LOM31_RS18785 (3798573)	3798573..3798941	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
LOM31_RS18790 (3799061)	3799061..3799714	+	654	Protein_3684	oxygen-insensitive NAD(P)H nitroreductase	-
LOM31_RS18795 (3799852)	3799852..3800139	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
LOM31_RS18800 (3800132)	3800132..3800449	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOM31_RS18805 (3800634)	3800634..3801677	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
LOM31_RS18810 (3802347)	3802347..3803213	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
LOM31_RS18815 (3803322)	3803322..3804749	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T225924 WP_004893639.1 NZ_CP087669:c3800449-3800132 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T225924 NZ_CP121579:c3326701-3326312 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT225924 WP_004142561.1 NZ_CP087669:c3800139-3799852 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT225924 NZ_CP121579:c3326965-3326708 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1