Detailed information of TA system TA225909

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 111128..111381
• Replicon: plasmid pDD01845-3
• Accession: NZ_CP087666
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01845

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LMH56_RS29590
• Protein ID: WP_001312851.1
• Coordinates: 111232..111381 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	111128..111187 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH56_RS29550 (106510)	106510..106854	-	345	Protein_148	IS6-like element IS26 family transposase	-
LMH56_RS29555 (106906)	106906..107610	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LMH56_RS29560 (107635)	107635..107835	+	201	WP_072354025.1	hypothetical protein	-
LMH56_RS29565 (107855)	107855..108601	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LMH56_RS29570 (108656)	108656..109216	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LMH56_RS29575 (109348)	109348..109548	+	201	WP_015059022.1	hypothetical protein	-
LMH56_RS29580 (109934)	109934..110533	+	600	WP_032083981.1	PIN domain-containing protein	-
LMH56_RS29585 (110595)	110595..110927	+	333	WP_152916585.1	hypothetical protein	-
- (111128)	111128..111187	-	60	NuclAT_1	-	Antitoxin
- (111128)	111128..111187	-	60	NuclAT_1	-	Antitoxin
- (111128)	111128..111187	-	60	NuclAT_1	-	Antitoxin
- (111128)	111128..111187	-	60	NuclAT_1	-	Antitoxin
LMH56_RS29590 (111232)	111232..111381	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LMH56_RS29595 (111665)	111665..111913	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..112224	112224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T225909 WP_001312851.1 NZ_CP087666:111232-111381 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T225909 NZ_CP121558:9288-9575 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT225909 NZ_CP087666:c111187-111128 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin