Detailed information of TA system TA225884

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 111294..111547
• Replicon: plasmid pDD01635-3
• Accession: NZ_CP087660
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01635

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LMH51_RS29945
• Protein ID: WP_001312851.1
• Coordinates: 111398..111547 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	111294..111353 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH51_RS29905 (106676)	106676..107020	-	345	Protein_148	IS6-like element IS26 family transposase	-
LMH51_RS29910 (107072)	107072..107776	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LMH51_RS29915 (107801)	107801..108001	+	201	WP_072354025.1	hypothetical protein	-
LMH51_RS29920 (108021)	108021..108767	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LMH51_RS29925 (108822)	108822..109382	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LMH51_RS29930 (109514)	109514..109714	+	201	WP_015059022.1	hypothetical protein	-
LMH51_RS29935 (110100)	110100..110699	+	600	WP_032083981.1	PIN domain-containing protein	-
LMH51_RS29940 (110761)	110761..111093	+	333	WP_152916585.1	hypothetical protein	-
- (111294)	111294..111353	-	60	NuclAT_1	-	Antitoxin
- (111294)	111294..111353	-	60	NuclAT_1	-	Antitoxin
- (111294)	111294..111353	-	60	NuclAT_1	-	Antitoxin
- (111294)	111294..111353	-	60	NuclAT_1	-	Antitoxin
LMH51_RS29945 (111398)	111398..111547	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LMH51_RS29950 (111831)	111831..112079	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..112390	112390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T225884 WP_001312851.1 NZ_CP087660:111398-111547 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T225884 NZ_CP121513:991288-991650 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT225884 NZ_CP087660:c111353-111294 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin