Detailed information of TA system TA225850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 60067..60794
• Replicon: plasmid pDD01653-1
• Accession: NZ_CP087652
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01653

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LL272_RS27215
• Protein ID: WP_011251285.1
• Coordinates: 60483..60794 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LL272_RS27210
• Protein ID: WP_011251286.1
• Coordinates: 60067..60486 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL272_RS27180 (LL272_27180)	55235..55705	-	471	WP_048333570.1	hypothetical protein	-
LL272_RS27185 (LL272_27185)	56018..56653	-	636	WP_223171879.1	hypothetical protein	-
LL272_RS27190 (LL272_27190)	57072..57692	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
LL272_RS27195 (LL272_27195)	57713..58501	+	789	WP_040217257.1	hypothetical protein	-
LL272_RS27200 (LL272_27200)	58515..58880	+	366	WP_048333448.1	hypothetical protein	-
LL272_RS27205 (LL272_27205)	58952..59920	-	969	WP_086642568.1	IS5 family transposase	-
LL272_RS27210 (LL272_27210)	60067..60486	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LL272_RS27215 (LL272_27215)	60483..60794	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LL272_RS27220 (LL272_27220)	60999..61436	-	438	Protein_60	DDE-type integrase/transposase/recombinase	-
LL272_RS27225 (LL272_27225)	61571..62268	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
LL272_RS27230 (LL272_27230)	62270..62719	+	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
LL272_RS27235 (LL272_27235)	62736..63047	+	312	WP_011251282.1	hypothetical protein	-
LL272_RS27240 (LL272_27240)	63061..63402	+	342	WP_011251281.1	hypothetical protein	-
LL272_RS27245 (LL272_27245)	63462..64418	+	957	WP_011251280.1	DsbA family protein	-
LL272_RS27250 (LL272_27250)	64843..65283	-	441	WP_011251275.1	hypothetical protein	-
LL272_RS27255 (LL272_27255)	65289..65783	-	495	WP_011251274.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..220277	220277
	-	inside	IScluster/Tn	-	-	43396..67703	24307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T225850 WP_011251285.1 NZ_CP087652:c60794-60483 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T225850 NZ_CP121476:c4002920-4002351 [Pseudomonas sp. 905_Psudmo1]
ATGTTCAAGCCGCAGCTGGTGACTTTGCAACGTGGCGCTCTGCGCCTGGAACCGCTCTCTGACGCGGATATCCCCGCGCT
GGTGGCGCTGGCCGAAGCCAACCGCGAGGAGCTGCTGTACATGAGCGGCACCCAGCGACTGGACTGGTACCGCAGCGCCC
TGGCCGATATGCGCGAGCAGCGGGCACTGCCGTTCGTCATTCGTCTGGGTGACGAGTTGGTAGGCACCACCCGCTTCGCC
GACTTCATGAACACCCTGCCGGCCTGTGAAATCGGCTGGACCTGGCTCGACCGCGGCCAGCATGGCAGCGGGTTGAACAC
CAGCATCAAGTACTTGATGCTCAAGCACGCTTTCGAAAATTGGGGCATGGTTCGCGTGCAACTGAAAACCGCCGCCAGCA
ACCTGCGTTCGCAACGCGCCATCGAGAAGCTTGGCGCCGTTCGTGAAGGGTTGCTGCGCAACCATCGCCGCCTGGCTGAT
GGCCGACTCGATGACACCGTGCTGTACAGCATCACCGACCTGGAATGGCCGGCGCTGCGTGGGGCAATGGAGGCGGGCTT
CAACGCCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT225850 WP_011251286.1 NZ_CP087652:c60486-60067 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT225850 NZ_CP121476:c4002345-4001518 [Pseudomonas sp. 905_Psudmo1]
ATGCACAGTCGCGAGCAGACCCGCTTCTGGCAAGCTTCCGCGTTGAGCGGCGTCGAGCTGTTGCAGGCGCGCTACCTTGA
ACAGCGCTTCGCGCCCCACGTGCATGAAGGTTTCGTGTTCACGGTGATCGAGCATGGCGCTCAGCGCTTTCGTCATCGCG
GTTGTGATCATCTGGCCCCACAGGGCAGCATGGTGCTGATCAATCCGGACGAAGTGCACACCGGCTCCAAGGCGCACGAA
GACGGATGGCGCTATCGCGGTTTTTATCCCGACAACGCCCAGGTACTGGGGGCGTTGCAGGAGTTGGGCCTGGCCAAGGC
TGGCATGCCGTCGTTTGCCTTCAGCGTGCTGCATGATCCACGTTTGCATGCGGCCTTTCTGCAACTGCACCAACTGCTCG
AGGGTGGTGCCGAGGCACTGCAGCAGCAACTGGCCTGGCGTGAGGCGATTCTGCTGCTGTTTCAGCACCATGCGCAGCTT
GCCGAACCACCTGCGCCTGGGCGTGAGCCCTGGGCAGTGGCTTGTGCCAAGCAGATGCTTGCCGAACGCCTGGTCGAGCC
GCCATCGCTTGAAGAGCTGGCTGCTGCGGTCAACCTCTCACCTTTTCACTTCGCGCGCGTATTTCGCCGCGCGACCGGCT
TGCCACCGCATGCCTGGCTCAAGCAGCGCCGCCTGGAGCAGGCGCGGGCGTTGCTCAAGACTGGCTGCACGGCGGTCGCT
GTGGCCATGCAGTTGGGCTTCGCCGATCAGAGCCACTTGTCGCGGCAGTTCAAGCAGGTTTATGGCGTGAGCCCTGGCGA
ATATCGCAGCGCGGTGGCCCGGAGCTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1