Detailed information of TA system TA225830

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 181460..181713
• Replicon: plasmid pDD01754-2
• Accession: NZ_CP087647
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01754

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LMH55_RS29800
• Protein ID: WP_001312851.1
• Coordinates: 181564..181713 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	181460..181519 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH55_RS29760 (177120)	177120..177185	-	66	Protein_230	helix-turn-helix domain-containing protein	-
LMH55_RS29765 (177238)	177238..177942	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LMH55_RS29770 (177967)	177967..178167	+	201	WP_072354025.1	hypothetical protein	-
LMH55_RS29775 (178187)	178187..178933	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LMH55_RS29780 (178988)	178988..179548	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LMH55_RS29785 (179680)	179680..179880	+	201	WP_015059022.1	hypothetical protein	-
LMH55_RS29790 (180266)	180266..180865	+	600	WP_032083981.1	PIN domain-containing protein	-
LMH55_RS29795 (180927)	180927..181259	+	333	WP_152916585.1	hypothetical protein	-
- (181460)	181460..181519	-	60	NuclAT_1	-	Antitoxin
- (181460)	181460..181519	-	60	NuclAT_1	-	Antitoxin
- (181460)	181460..181519	-	60	NuclAT_1	-	Antitoxin
- (181460)	181460..181519	-	60	NuclAT_1	-	Antitoxin
LMH55_RS29800 (181564)	181564..181713	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LMH55_RS29805 (181997)	181997..182245	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..182556	182556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T225830 WP_001312851.1 NZ_CP087647:181564-181713 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T225830 NZ_CP121375:c111749-111486 [Acinetobacter baumannii]
ATGCATGTATTTACATATACAGACGCACGTAACAACTTAAAATCTGTTTTAGATAAAGTTATTGATGATGCAGATGTCGC
TTTCATTACAAGAAAAGAAGGCGGTCATGCTGTAGTAATGGGACAAGATCATTACGATAGTTTAATGGAAACGTTGTATC
TCCTTTCATCTCCAAATAATACCACTCGTTTAAACGAATCAATTGCTCAATTACGCGCAGGTAAAACCAAACAAAGAGAG
TTGATTAAAGATGACGAGTCGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT225830 NZ_CP087647:c181519-181460 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin