Detailed information of TA system TA225826

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51676..51945
• Replicon: plasmid pDD01754-2
• Accession: NZ_CP087647
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01754

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LMH55_RS28945
• Protein ID: WP_001372321.1
• Coordinates: 51820..51945 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	51676..51741 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH55_RS28915	47386..47913	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LMH55_RS28920	47971..48204	+	234	WP_000006003.1	DUF905 family protein	-
LMH55_RS28925	48265..50288	+	2024	Protein_63	ParB/RepB/Spo0J family partition protein	-
LMH55_RS28930	50357..50791	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LMH55_RS28935	50788..51507	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	51519..51743	+	225	NuclAT_0	-	-
-	51519..51743	+	225	NuclAT_0	-	-
-	51519..51743	+	225	NuclAT_0	-	-
-	51519..51743	+	225	NuclAT_0	-	-
-	51676..51741	-	66	-	-	Antitoxin
LMH55_RS28940	51729..51878	+	150	Protein_66	plasmid maintenance protein Mok	-
LMH55_RS28945	51820..51945	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LMH55_RS28950	52264..52560	-	297	Protein_68	hypothetical protein	-
LMH55_RS28955	52860..53156	+	297	WP_001272251.1	hypothetical protein	-
LMH55_RS28960	53267..54088	+	822	WP_001234445.1	DUF932 domain-containing protein	-
LMH55_RS28965	54385..55032	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
LMH55_RS28970	55309..55692	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LMH55_RS28975	55883..56569	+	687	WP_015059009.1	PAS domain-containing protein	-
LMH55_RS28980	56663..56890	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..182556	182556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T225826 WP_001372321.1 NZ_CP087647:51820-51945 [Klebsiella pneumoniae subsp. pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T225826 NZ_CP121373:c17054-16734 [Acinetobacter baumannii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGGA
AGATCTTTTGGTACAGCCTGATAGAGGTGATCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGAAAAAGTGGTGGTATTCGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATATTTTTCTTATTGGCTTAT
CCAAAATCAGTGAAAGATAATTTAACAGATAAGGAAACAGCCATTCTGCGCCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Nucleotide

• Length: 66 bp

>AT225826 NZ_CP087647:c51741-51676 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin