Detailed information of TA system TA225804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 40316..41043
• Replicon: plasmid pDD02391-2
• Accession: NZ_CP087641
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02391

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LMH57_RS28145
• Protein ID: WP_011251285.1
• Coordinates: 40732..41043 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LMH57_RS28140
• Protein ID: WP_011251286.1
• Coordinates: 40316..40735 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH57_RS28110 (LMH57_28110)	35484..35954	-	471	WP_048333570.1	hypothetical protein	-
LMH57_RS28115 (LMH57_28115)	36267..36902	-	636	WP_223171879.1	hypothetical protein	-
LMH57_RS28120 (LMH57_28120)	37321..37941	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
LMH57_RS28125 (LMH57_28125)	37962..38750	+	789	WP_040217257.1	hypothetical protein	-
LMH57_RS28130 (LMH57_28130)	38764..39129	+	366	WP_048333448.1	hypothetical protein	-
LMH57_RS28135 (LMH57_28135)	39201..40169	-	969	WP_086642568.1	IS5 family transposase	-
LMH57_RS28140 (LMH57_28140)	40316..40735	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LMH57_RS28145 (LMH57_28145)	40732..41043	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LMH57_RS28150 (LMH57_28150)	41248..41685	-	438	Protein_46	DDE-type integrase/transposase/recombinase	-
LMH57_RS28155 (LMH57_28155)	41820..42517	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
LMH57_RS28160 (LMH57_28160)	43903..44553	-	651	WP_068893702.1	DUF1173 family protein	-
LMH57_RS28165 (LMH57_28165)	44586..44852	-	267	WP_223175074.1	DUF1173 family protein	-
LMH57_RS28170 (LMH57_28170)	45030..45524	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	rmpA / iroN	1..132733	132733
	-	inside	IScluster/Tn	-	-	22368..47710	25342

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T225804 WP_011251285.1 NZ_CP087641:c41043-40732 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T225804 NZ_CP121358:42579-42959 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCTAATATAAACCGCTACGGCGGCTTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT225804 WP_011251286.1 NZ_CP087641:c40735-40316 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT225804 NZ_CP121358:42358-42579 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1