Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 132676..132929 Replicon plasmid pDD02391-1
Accession NZ_CP087640
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02391

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag LMH57_RS27910 Protein ID WP_001312851.1
Coordinates 132780..132929 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 132676..132735 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH57_RS27870 (128058) 128058..128402 - 345 Protein_162 IS6-like element IS26 family transposase -
LMH57_RS27875 (128454) 128454..129158 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LMH57_RS27880 (129183) 129183..129383 + 201 WP_072354025.1 hypothetical protein -
LMH57_RS27885 (129403) 129403..130149 + 747 WP_000205770.1 conjugal transfer pilus acetylase TraX -
LMH57_RS27890 (130204) 130204..130764 + 561 WP_000139328.1 fertility inhibition protein FinO -
LMH57_RS27895 (130896) 130896..131096 + 201 WP_015059022.1 hypothetical protein -
LMH57_RS27900 (131482) 131482..132081 + 600 WP_032083981.1 PIN domain-containing protein -
LMH57_RS27905 (132143) 132143..132475 + 333 WP_152916585.1 hypothetical protein -
- (132676) 132676..132735 - 60 NuclAT_1 - Antitoxin
- (132676) 132676..132735 - 60 NuclAT_1 - Antitoxin
- (132676) 132676..132735 - 60 NuclAT_1 - Antitoxin
- (132676) 132676..132735 - 60 NuclAT_1 - Antitoxin
LMH57_RS27910 (132780) 132780..132929 + 150 WP_001312851.1 Hok/Gef family protein Toxin
LMH57_RS27915 (133213) 133213..133461 + 249 WP_000083837.1 replication regulatory protein RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 - 1..133772 133772


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T225802 WP_001312851.1 NZ_CP087640:132780-132929 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T225802 NZ_CP121356:c4766273-4766100 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 60 bp

>AT225802 NZ_CP087640:c132735-132676 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References