Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | hok-sok/- |
| Location | 75011..75264 | Replicon | plasmid pDD02297-1 |
| Accession | NZ_CP087635 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain DD02297 | ||
Toxin (Protein)
| Gene name | srnB | Uniprot ID | G9G1E3 |
| Locus tag | LMH52_RS27665 | Protein ID | WP_001312851.1 |
| Coordinates | 75115..75264 (+) | Length | 50 a.a. |
Antitoxin (RNA)
| Gene name | srnC | ||
| Locus tag | - | ||
| Coordinates | 75011..75070 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LMH52_RS27620 (70044) | 70044..70349 | + | 306 | WP_004144424.1 | type IV conjugative transfer system protein TraL | - |
| LMH52_RS27625 (70369) | 70369..70734 | + | 366 | Protein_97 | type IV conjugative transfer system protein TraE | - |
| LMH52_RS27630 (70789) | 70789..71493 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| LMH52_RS27635 (71518) | 71518..71718 | + | 201 | WP_072354025.1 | hypothetical protein | - |
| LMH52_RS27640 (71738) | 71738..72484 | + | 747 | WP_000205770.1 | conjugal transfer pilus acetylase TraX | - |
| LMH52_RS27645 (72539) | 72539..73099 | + | 561 | WP_000139328.1 | fertility inhibition protein FinO | - |
| LMH52_RS27650 (73231) | 73231..73431 | + | 201 | WP_015059022.1 | hypothetical protein | - |
| LMH52_RS27655 (73817) | 73817..74416 | + | 600 | WP_032083981.1 | PIN domain-containing protein | - |
| LMH52_RS27660 (74478) | 74478..74810 | + | 333 | WP_152916585.1 | hypothetical protein | - |
| - (75011) | 75011..75070 | - | 60 | NuclAT_0 | - | Antitoxin |
| - (75011) | 75011..75070 | - | 60 | NuclAT_0 | - | Antitoxin |
| - (75011) | 75011..75070 | - | 60 | NuclAT_0 | - | Antitoxin |
| - (75011) | 75011..75070 | - | 60 | NuclAT_0 | - | Antitoxin |
| LMH52_RS27665 (75115) | 75115..75264 | + | 150 | WP_001312851.1 | Hok/Gef family protein | Toxin |
| LMH52_RS27670 (75548) | 75548..75796 | + | 249 | WP_000083837.1 | replication regulatory protein RepA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaCTX-M-65 / blaKPC-2 | - | 1..76107 | 76107 | |
| - | flank | IS/Tn | - | - | 70789..71493 | 704 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 50 a.a. Molecular weight: 5542.67 Da Isoelectric Point: 8.7678
>T225777 WP_001312851.1 NZ_CP087635:75115-75264 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK
Download Length: 150 bp
>T225777 NZ_CP121355:c4775763-4775506 [Mesorhizobium sp. WSM4906]
ATGAAGATCGTTTGGGATGAACCGAAACGGTTGGCTAACATCGACAAACACGGCTTGGATTTCGCGGCGTTGGACGAGGA
GTTTTTCCTTGCGTCGACGATCCGGGCGGCCAAGGCCGGTCGGTTCATGGCGATTGGTTGGATAGTCGGCGGCGTTGTTG
CAGTGGTCTTTGCCCGACTTGGCTCCGAGGGCATTTCGATCATTTCGATGCGGCCGGCCAACCAGGCGGAAAGGAGATTG
TTCGATGGCAAAGTCTAA
ATGAAGATCGTTTGGGATGAACCGAAACGGTTGGCTAACATCGACAAACACGGCTTGGATTTCGCGGCGTTGGACGAGGA
GTTTTTCCTTGCGTCGACGATCCGGGCGGCCAAGGCCGGTCGGTTCATGGCGATTGGTTGGATAGTCGGCGGCGTTGTTG
CAGTGGTCTTTGCCCGACTTGGCTCCGAGGGCATTTCGATCATTTCGATGCGGCCGGCCAACCAGGCGGAAAGGAGATTG
TTCGATGGCAAAGTCTAA
Antitoxin
Download Length: 60 bp
>AT225777 NZ_CP087635:c75070-75011 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
